Cancer Biomarkers - Volume 25, issue 2

Authors: Lampropoulou, Dimitra-Ioanna | Aravantinos, Gerasimos | Katifelis, Hector | Lazaris, Foivos | Laschos, Konstantinos | Theodosopoulos, Theodosios | Papadimitriou, Christos | Gazouli, Maria

Article Type: Research Article

Abstract: BACKGROUND : Colorectal cancer is the fourth cause of cancer related death. Drug resistance and toxicity remain major clinical issues. HOTAIR and MALAT1 are long non-coding RNAS that affect cellular proliferation, apoptosis and drug resistance; their up-regulation has been linked with a poor prognosis. OBJECTIVE: Investigation of the association between rs4759314 HOTAIR and rs3200401 MALAT1 polymorphisms and irinotecan-based chemotherapy in terms of drug efficacy and toxicity. METHODS: Samples from 98 patients receiving different regimens of irinotecan-based therapy were included. Efficacy and toxicity were evaluated. KRAS mutation, rs3200401 HOTAIR …and rs4759314 MALAT1 polymorphisms genotyping in the tumors and peripheral blood respectively were performed with PCR. RESULTS: Neither rs3200401 MALAT1 nor rs4759314 HOTAIR polymorphism are associated with response to treatment regimens. Rs4759314 was also not associated with increased toxicity in patients receiving irinotecan-based regimens. CT genotype of rs3200401 was associated with significantly reduced overall survival. An association between KRAS mutation and AG/GG genotypes in the rs4759314 was detected. CONCLUSIONS: CT genotype of rs3200401 MALAT1 polymorphism could serve as a toxicity biomarker. Carriers of the G allele of the rs4759314 HOTAIR are more likely to be carriers of KRAS mutations too. However, further studies in larger patient populations are required. Show more

Keywords: CRC, lncRNA, HOTAIR, MALAT1, rs3200401, rs4759314 irinotecan, drug resistance, toxicity

DOI: 10.3233/CBM-182383

Citation: Cancer Biomarkers, vol. 25, no. 2, pp. 213-221, 2019

Authors: Gondkar, Kirti | Patel, Krishna | Krishnappa, Shobha | Patil, Akkamahadevi | Nair, Bipin | Sundaram, Gopinath Meenakshi | Zea, Tan Tuan | Kumar, Prashant

Article Type: Research Article

Abstract: BACKGROUND: Transcription factors are commonly deregulated in various cancers. Here, we evaluated role of ELF3 in pathogenesis of bladder carcinoma (BCa). MATERIALS AND METHODS: We confirmed ELF3 expression pattern in BCa cell lines using western blot; and in different grades of tumors using Immunohistochemistry. Cell invasion assay was employed to demonstrate potential role of ELF3 in EMT. RESULTS AND CONCLUSION: ELF3 showed selective expression in low-grade cell lines and tumor tissues. Overexpression of ELF3 in mesenchymal cell line UMUC3 resulted in reduced invasion and decreased expression of mesenchymal markers. We observed association of …low ELF3 expression with increased risk and overall poor survival using publicly available data. ELF3-modulated reversal of EMT might be a useful strategy in the treatment of bladder cancer. Show more

Keywords: EMT, bladder cancer, ETS transcription factor

DOI: 10.3233/CBM-190013

Citation: Cancer Biomarkers, vol. 25, no. 2, pp. 223-232, 2019