Cancer Biomarkers - Volume 21, issue 1

Authors: Yuan, Linjing | Wan, Jianxin | Huang, Chumei | Liang, Jingjing | Liu, Min | Yue, Caifeng | Li, Laisheng

Article Type: Research Article

Abstract: BACKGROUND: Early detection and differentiation diagnosis of a pelvic mass (PM) is crucial in improving the prognosis of patients with epithelial ovarian cancer (EOC). C-C motif chemokine ligand 18 (CCL18) was reported as a chemokine-mediated tumor-related inflammation that can be detected in serum and may correlate with cancer patients’ prognosis. OBJECTIVE: We performed this study to investigate the relationship between CCL18 levels and clinical characteristics of EOC patients, and to explore their diagnostic and prognostic values. METHODS: CCL18 serum concentrations were detected by ELISA in 187 patients with EOC, 126 patients with benign …PMs, and 118 healthy controls. CCL18 serum levels were analyzed in the context of patients’ clinicopathological information, and ROC analyses were performed to determine the effect of CCL18 on distinguishing benign and malignant PMs. The ability of CCL18 to serve as an EOC biomarker was compared with CA125. Further survival analyses were carried out to assess the prognostic value of CCL18 in EOC patients. RESULTS: Mean serum CCL18 levels were elevated in benign PM patients and were even higher in EOC patients than in healthy controls; furthermore, high CCL18 expression was associated with worse International Federation of Gynecology and Obstetrics (FIGO) staging and predicted a poorer survival of the patient. When compared with CA125, although the sensitivity and negative predictive values (NPV) of serum CCL18 were lower, its specificity and positive predictive values (PPV) were higher. CONCLUSIONS: Serum CCL18 was elevated in patients with EOC and could serve as a new tumor biomarker, which also predicted a poor survival of the patient. Show more

Keywords: CCL18, ovarian cancer, prognosis, biomarker, CA125

DOI: 10.3233/CBM-170305

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 97-104, 2018

Authors: Yu, Liang | Chen, Jun | Liu, Yu | Zhang, Zengfeng | Duan, Shaobin

Article Type: Research Article

Abstract: This article has been retracted, and the online PDF replaced with this retraction notice.

Keywords: miR-937, FOXL2, gastric cancer, proliferation, invasion

DOI: 10.3233/CBM-170310

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 105-116, 2018

Authors: Liu, Suoning | Suo, Jian | Wang, Chunxi | Sun, Xuan | Wang, Daguang | He, Liang | Zhang, Yang | Li, Wei

Article Type: Research Article

Abstract: Numerous studies have proved that microRNAs (miRNAs) play crucial roles in the tumorigenesis and progression of gastric cancer (GC). Our study was to investigate the correlation between miR-338-3p expression and clinical features as well as prognosis of GC. A total of 138 GC tissue specimens and adjacent non-cancerous tissues were collected for further analysis, then quantitative PCR method was used to detect the relative miR-338-3p expression. Our study showed that tissue miR-338-3p level was greatly decreased in cancer tissues compared with paired normal tissues. Furthermore, loss of tissue miR-338-3p was positively associated with aggressive clinical characteristics (advanced clinical stage, poorer …differentiation and lymph node invasion), shorter overall survival and recurrence free survival. Finally, tissue miR-338-3p expression was confirmed to be an independent prognostic factor for GC. Overall, our findings indicate that miR-338-3p acts as a tumor suppressor in GC and tissue miR-338-3p might serve as a novel prognostic biomarker of GC. Show more

Keywords: Gastric cancer, miR-338-3p, prognosis, biomarker

DOI: 10.3233/CBM-170339

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 117-122, 2018

Authors: Xie, Pengmu | Cao, Hongying | Li, Ying | Wang, Jianhua | Cui, Zhumei

Article Type: Research Article

Abstract: This article has been retracted, and the online PDF has been watermarked ``RETRACTION''. The retraction notice is available at http://doi.org/10.3233/CBM-229010.

Keywords: LncRNA colon cancer-associated transcript 2 (CCAT2), endometrial cancer, miR-216b, Bcl-2, PTEN/PI3K/AKT and mTOR signaling pathways

DOI: 10.3233/CBM-170388

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 123-133, 2018

Authors: Li, Qi-Cai | Xu, Haiyan | Wang, Xiaohui | Wang, Ting | Wu, Jiang

Article Type: Research Article

Abstract: BACKGROUND AND OBJECTIVE: Osteosarcoma is the most common primary malignancy in bone. Patients who respond poorly to induction chemotherapy are at higher risk of adverse prognosis. The molecular basis for such poor prognosis remains unclear. Recently, increasing evidence has suggested decreased expression of miR-34a is observed in a number of cancer types, including human osteosarcoma, and decreased miR-34a is involved in drug resistance. However, the underlying molecular mechanisms of decreased miR-34a on cisplatin chemoresistance in osteosarcoma has not been reported. METHODS: Osteosarcoma U2OS cells were transfected with miR-34a mimics for 48 h, then the cells were …treated with 3.0 μ m cisplatin for 24 h. Using siRNA targeting c-Myc and Bim to examine the relation between miR-34a, c-Myc and Bim expression exposure to cisplatin on cisplatin-induced apoptosis. RESULTS: Treatment of U2OS cells with cisplatin induced cell apoptosis by upregulation of c-Myc -dependent Bim expression; Osteosarcoma U2OS cells transfected with miR-34a mimics (miR-34a/U2OS) induced cell apoptosis and inhibited cell survival, and increased the sensitivity of U2OS cells to cisplatin. U2OS cells transfected with miR-34a mimics upregulated the protein expression of c-Myc and Bim. Targeting c-Myc downregulated the expression of Bim in the miR-34a/U2OS cells. In addition, Targeting Bim reversed the chemeresistance of miR-34a/U2OS cells to cisplatin. CONCLUSIONS: Our data indicated that miR-34a enhanced the sensitivity to cisplatin by upregulation of c-Myc and Bim pathway. Show more

Keywords: Osteosarcoma, chemotherapy, cisplatin, miR-34a, c-Myc, Bim

DOI: 10.3233/CBM-170452

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 135-144, 2018

Authors: Luo, Lan | Xu, Lina | Tang, Liang

Article Type: Research Article

Abstract: Endometrial carcinoma (EC) is a common malignant tumor in gynecology. Its incidence and development are closely associated with the levels of estrogenic and progesterone hormone. Extracellular signal-regulated kinase (ERK) signaling pathway abnormity is associated with a variety of tumors. This study detected estrogen receptor (ER), progesterone receptor (PR), ERK1, and ERK2 expression in EC and analyzed their correlations. A total of 40 EC patients in our hospital were selected as test group, while another 40 healthy volunteers were enrolled as control group. ER, PR, ERK1, and ERK2 expression in EC tissue, para-carcinoma tissue, and normal endometrial tissue were detected by …immunohistochemistry and Western blot. The positive rate of ER, PR, ERK1, and ERK2 in the test group was 50%, 40%, 60%, and 65%, respectively, which were significantly higher than those in the control (P < 0.05). ER, PR, ERK1, and ERK2 protein expressions in EC cell were significantly higher than those in the control (P < 0.05). ERK1 and ERK2 presented positive correlation with ER and PR (P < 0.05). In conclusion, EC patients presented higher expressions of ER, PR, which were correlated with higher levels of ERK1 and ERK2, suggesting they might be involved in the pathogenesis of EC. Show more

Keywords: Endometrial carcinoma, ER, PR, ERK1, ERK2

DOI: 10.3233/CBM-170457

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 145-149, 2018

Authors: Lian, Dongbo | Amin, Buhe | Du, Dexiao | Yan, Wei

Article Type: Research Article

Abstract: This paper aimed to probe into the expression of long non-coding RNA (lncRNA) SNHG16 in human gastric cancer (GC) and its potential tumor biological functions. The expression of lncRNA SNHG16 was detected in GC and adjacent tissues and GC cell lines using qRT-PCR. GC MGC-803 cells were transfected with siRNA of lncRNA SNHG16, as well as blank and negative control. A series of experiments including CCK-8, flow cytometry, transwell, and wound healing assay were adopted to evaluate the effects of lncRNA SNHG16 on cell growth and metastasis. Besides, the nude mouse xenograft tumor model was established to draw tumor growth …curve and measure tumor volume during treatments. TUNEL staining was used to determine the apoptosis rate of tissues. The expression of lncRNA SNHG16 in GC tissue, significantly associated with invasion depth, lymph node metastasis, TNM stage and histological differentiation (all P < 0.05), was upregulated compared with adjacent tissues. Transfected with siRNA of lncRNA SNHG16 inhibited GC MGC-803 cell proliferation, and arrested cells in the G0/G1 phase, and then promoted apoptosis rate with reduced cell invasion and shortened migration distance. Additionally, the nude mice xenograft presented lower tumor growth rate and weight loss alongside elevated apoptosis rate of tumor tissues. LncRNA SNHG16 is highly expressed in GC, while suppression of SNHG16 expression can inhibit proliferation, weaken invasion and migration of GC cells, and enhance apoptosis, to be a novel target for GC clinical treatment. Show more

Keywords: Long non-coding RNA SNHG16, gastric cancer, clinicopathological features, proliferation, migration, invasion, apoptosis, cell cycle

DOI: 10.3233/CBM-170462

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 151-160, 2018

Authors: Lu, Nanhang | Wang, Jinzeng | Zhu, Bijun | Zhang, Miaomiao | Qi, Fazhi | Wang, Xiangdong | Gu, Jianying

Article Type: Research Article

Abstract: BACKGROUND: Nevoid basal cell carcinoma syndrome (NBCCS) is a rare autosomal dominant disease with a complex genetic etiology. Although three causative genes (PTCH1, PTCH2, SUFU) have been identified through linkage analysis and Sanger sequencing, the genetic background of NBCCS hasn’t been fully understood. METHODS: We performed a whole-exome sequencing (WES) in a Han Chinese NBCCS family and two unaffected volunteers to search for its causative gene. Bioinformatic analysis was used to select candidate genes and analyze the functional networks of each candidate gene. RESULTS: A total of 8 single-nucleotide variants (SNVs) were detected …in PTCH1, PTCH2 and SUFU in all the 5 subjects, however none of them was considered the pathogenic genetic mutation in this NBCCS family. The following filtering process identified 17 novel candidate genes (GBP3, AMPD1, ASPM, UNC5C, RBM46, HSPA1L, PNPLA1, GPR126, AP5Z1, ZFHX4, KIF24, C10orf128, COX15, GPRC5A, UGGT2, RHBDF1, RPUSD1). Among them ZFHX4 had been already identified as a new basal cell carcinoma susceptibility loci through a genome-wide association study (GWAS) and was considered the most likely pathogenic gene for this NBCCS family. The functional network analysis revealed that ZFHX4 may be involved in notch signaling pathway. CONCLUSIONS: Our study reported the identification of 17 novel candidate genes in a Han Chinese family through WES. ZFHX4 may be a susceptibility gene for NBCCS in Chinese population. Show more

Keywords: Gorlin syndrome, nevoid basal cell carcinoma syndrome, whole exome sequencing, Single-nucleotide variants, ZFHX4

DOI: 10.3233/CBM-170541

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 161-168, 2018

Authors: Zhao, Xiaoliang | Wen, Xiaohua | Wei, Wei | Su, Yanjun | You, Jian | Gong, Liqun | Zhang, Zhenfa | Wang, Meng | Xiao, Jianyu | Wei, Xiyin | Wang, Changli

Article Type: Research Article

Abstract: BACKGROUND: Endostar (rh-endostatin) is a new recombinant human endostatin, which could inhibit cell proliferation, angiogenesis, and tumor growth. OBJECTIVE: To explore anti-angiogenesis short-term efficacy combined with neoadjuvant chemotherapy for stage IIIA (N2) non-small cell lung cancer (NSCLC), and identify the potential predictive factors. METHODS: We pathologically examined 26 patients diagnosed with stage IIIA (N2) NSCLC who received NP chemotherapy alone or combined with Endostar, respectively. RESULTS: Our results indicated that total clinical benefit rate (CBR) 87.5% and 64% (p = 0.76), respectively. The clinical benefit …(CB) patients in the treatment group showed significant changes in endothelial progenitor cells (EPC), vascular endothelial growth factor (VEGF), blood flow (BF), permeability surface (PMS), and microvascular density (MVD) before and after treatment. Compared with CB patients in the control group, changes in EPC and MVD (only) before and after treatment were significant. The variation of EPC, PMS, and MVD before and after treatment in the treatment group showed positive correlation with tumor regression rate (TRR) and the variation of MVD, whereas those of EPC and PMS demonstrated positive correlations with variation of MVD before and after treatment. CONCLUSION: Our findings suggested that PMS and EPC may be used as a predictive factor for the short-term efficacy of the combined therapy in NSCLC. Show more

Keywords: RH-endostatin, non-small cell lung cancer, predictor, circulating endothelial progenitor cells, perfusion imaging

DOI: 10.3233/CBM-170565

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 169-177, 2018

Authors: Zhang, Suxia | Wang, Min | Li, Qirong | Zhu, Ping

Article Type: Research Article

Abstract: This article has been retracted, and the online PDF replaced with this retraction notice.

Keywords: miR-101, PI3K/Akt/mTOR, apoptosis, invasion, proliferation, endometrial cancer

DOI: 10.3233/CBM-170620

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 179-186, 2018

Authors: Hou, Li | Hou, Xiaofei | Wang, Lijing | Li, Zenghui | Xin, Beibei | Chen, Jing | Gao, Xiaofei | Mu, Haixia

Article Type: Research Article

Abstract: BACKGROUND: The study was aimed at investigating the role of PD98059 on impairing the cisplatin-resistance of ovarian cancer cells and figuring out the potential mechanism. MATERIAL AND METHODS: Treated with low dose of cisplatin (DDP), DDP-resistant ovarian cancer cells were built and named as SKOV-3/DDP. The cell viabilities of ovarian cancer cell line SKOV-3 and SKOV-3/DDP were detected using MTT assay. Wound healing assay and flow cytometry were performed to detect the migratory ability and cell cycle variation of the two cells and assess the sensibility to DDP in the two cell lines. However, cotreated with DDP …and PD98059, cell viability, migration and cell cycle of SKOV-3/DDP were determined again. The DDP-resistance varied a lot and the potential mechanism was studied via western blot assay. RESULTS: Both treated with DDP, SKOV-3/DDP showed an intense resistance than SKOV-3 including stronger cell viability, larger migration area and less G1/G0 arrest, which confirmed the successfully established DDP-resistant cell line. The phosphorylation of ERK and the activation of epithelial mesenchymal transition (EMT) process contributes to the enhanced resistance. PD98059, a MEK inhibitor, suppresses the ERK pathway and the EMT process of SKOV-3/DDP. Co-treated by DDP and PD98059, cell proliferation and migratory area decreased, meantime more cell were arrested in G0/G1 phase compared to simple treatment of DDP or PD98059. CONCLUSION: PD98059 efficiently impairs the DDP-resistance of ovarian cancer cells via downregulating the ERK pathway and the EMT process. Show more

Keywords: Ovarian cancer, cisplatin resistance, epithelial mesenchymal transition, ERK pathway

DOI: 10.3233/CBM-170644

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 187-194, 2018

Authors: Hu, Xiao-Yan | Liu, Zhe | Zhang, Kai-Lin | Feng, Jing | Liu, Xiao-Fang | Wang, Ling-Yun | Wang, Zi-Wei

Article Type: Research Article

Abstract: N-myc downstream regulated gene 2 (NDRG2) is frequently down-regulated in various cancers and functions as a candidate tumor suppressor gene. NDRG2 has been shown to be SUMOylated on the lysine 333 residue, which promoted its ubiquitination and sequentially degradation by the SUMO-targeted ubiquitin E3 ligase RNF4. However, how to regulated NDRG2 deSUMOylation process remains largely unknown. Here, we report that Sentrin/SUMO specific protease (SENP2) was down-regulated in clinic gastric cancer samples and possessed a tumor-suppressive role in gastric cancer. At the molecular level, we found that SENP2 interacts with NDRG2 and mediates the de-SUMOylation process of NDRG2. Overexpression of SENP2 …stabilized NDRG2, whereas silencing SENP2 caused rapid NDRG2 SUMOylation and degradation, indicating SENP2 antagonizes NDRG2 ubiquitination and degradation, thereby promoting the stability and function of this protein. Thus, our study reveals that SENP2 acts as a tumor suppressor which is deregulated in gastric cancer and the specific de-SUMOylation activity of SENP2 for NDRG2 is critical for it stabilization as well as gastric cancer cells proliferation. Show more

Keywords: SENP2, NDRG2, gastric cancer, SUMOylation

DOI: 10.3233/CBM-170651

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 195-201, 2018

Authors: Sun, Jiachun | Wang, Dengkui | Li, Xiangming | Yan, Junqiang | Yuan, Xiaozhi | Wang, Wei

Article Type: Research Article

Abstract: OBJECTIVE: Glioma-associated oncogene homolog 1 (Gli1 ) in Hedgehog signal pathway regulates Cyclin D1 expression, cell cycle or proliferation modulation. Esophageal cancer patients had significantly elevated Gli1 expression, which is related with survival and prognosis. It has been demonstrated that the level of miR-150 was decreased in esophageal cancer patients compared to normal control. As a complementary relationship exists between miR-150 and 3’-UTR of Gli1 , this study investigated if miR-150 played a role in regulating Gli1 expression, and proliferation or cell cycle of esophageal cancer cells. PATIENTS AND METHODS: Esophageal squamous cell carcinoma (ESCC) …patients from our hospital were recruited to collect tumor and adjacent tissues for miR-150 and Gli1 expression. Esophageal carcinoma cell line EC9706 and normal esophageal epithelial cell line HEEC were compared for expression of miR-150, Gli1 and Cyclin D1 . Dual luciferase reporter gene assay examined the targeted relationship between miR-150 and 3’-UTR of Gli1 . In vitro cultured EC9706 cells were treated with miR-150 mimic, si-Gli1 or the combination of miR-150 mimic and si-Gli1, respectively, to check their gene expression, cell cycle and proliferation. RESULTS: ESCC tissues had significantly higher Gli1 expression and lower miR-150 expression. EC9706 cell also had higher Gli1 expression than that in HEEC, whilst miR-150 was down-regulated. Via targeting 3’-UTR of Gli1 gene, miR-150 inhibited its expression. Transfection of miR-150 mimic, si-Gli1 or the combination of miR-150 mimic and si-Gli1, respectively, remarkably decreased expression of Gli1 and Cyclin D1 expression in EC9706 cells, whose cell cycle arresting at G0/G1 phase was enhanced with weakened proliferation. CONCLUSIONS: MiR-150 can induce G0/G1 cell cycle arresting and weaken proliferation of esophageal carcinoma cells via targeted inhibition on Gli1 and downstream expression of Cyclin D1 . Show more

Keywords: MicroRNA-150, Gli1, Cyclin D1, cell proliferation, cell cycle, esophageal carcinoma

DOI: 10.3233/CBM-170658

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 203-210, 2018

Authors: Yang, Ching-Yao | Lin, Mong-Wei | Chang, Yih-Leong | Wu, Chen-Tu

Article Type: Research Article

Abstract: BACKGROUND: Globo H is a tumor-associated carbohydrate antigen exclusively expressed in cancer cells rather than normal tissue. Globo H has been found on many cancers of epithelial origins, and become an attractive target for cancer vaccine. OBJECTIVES: We aimed to study the expression of Globo H in non-small cell lung cancer (NSCLC) patients, and correlated its expression with common driver mutations, clinical outcomes, and status of immune checkpoint, programmed death-ligand 1 (PD-L1). METHODS: The study enrolled 228 patients with surgically resected stage I NSCLC, including 139 patients with adenocarcinoma (ADC) and 89 patients …with squamous cell carcinoma (SqCC). Using immunohistochemistry, tumors with moderate to strong membranous staining in ⩾ 1% tumor cells per section were scored as positive Globo H expression. Driver mutations including EGFR, KRAS, BRAF were detected by direct sequencing, while ALK, PI3KCA, FGFR1 and PD-L1 expression was detected by immunohistochemical (IHC) staining. RESULTS: Positive Globo H expression was detected in 88 of the 228 (38.6%) patients. These included 51 of 139 (36.7%) patients with ADC and 37 of 89 (41.6%) patients with SqCC. Positive Globo H expression was significantly associated with EGFR mutation and PD-L1 expression in the ADC group, and PI3KCA overexpression in the SqCC group. The survival analysis showed that Globo H expression was not an independent prognostic factor in stage I NSCLC. CONCLUSIONS: Globo H expression was correlated with specific driver mutations in ADC and SqCC NSCLC tumors, as well as PD-L1 status. Immunotherapy targeting Globo H may have potential application in lung cancer treatment. Show more

Keywords: Cancer vaccine, Globo H, immunotherapy, non-small cell lung cancer, tumor-associated carbohydrate antigen

DOI: 10.3233/CBM-170660

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 211-220, 2018

Authors: Huang, Tonghai | Wang, Guangsuo | Yang, Lin | Peng, Bin | Wen, Yuxin | Ding, Guanggui | Wang, Zheng

Article Type: Research Article

Abstract: BACKGROUND: Non-small cell lung cancer (NSCLC) is the main type of lung cancer. While miR-186 is significantly reduced in lung cancer tissues and cells, its role in NSCLC has not been completely elucidated. MATERIAL AND METHODS: We used qRT-PCR and western blot methods to investigate the levels of miR-186 and YY1 in 21 pairs of NSCLC tissues. Dual luciferase reporter gene assays were performed to detect whether miR-186 directly targets YY1 . Next, the roles of miR-186 and its target gene (YY1 ) in determining the proliferation, apoptosis and migration capabilities of selected cell lines (A549 and HCC827) …were investigated by using miR-186 mimics or YY1 siRNA. RESULTS: Our results showed that miR-186 was downregulated and YYI was upregulated in NSCLC tissue, and miR-186 expression was negatively associated with YY1. Similarly, miR-186 was also downregulated and YY1 expression also was upregulated in both A549 and HCC827 cells; furthermore, miR-186 was found to directly target YY1 . Cell proliferation, invasion, and migration, as well as apoptosis induction were more strongly inhibited by YY1 siRNA than by miR-186. CONCLUSION: Our results suggest that miR-186 and its target gene (YY1 ) could possibly serve as new prognostic biomarkers and therapeutic targets for treating NSCLC in humans. Show more

Keywords: miR-186, YY1, cell proliferation, apoptosis, migration and invasion, non-small cell lung cancer

DOI: 10.3233/CBM-170670

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 221-228, 2018

Authors: Feferman, Leo | Deaton, Ryan | Bhattacharyya, Sumit | Xie, Hui | Gann, Peter H. | Melamed, Jonathan | Tobacman, Joanne K.

Article Type: Research Article

Abstract: BACKGROUND: Arylsulfatase B (ARSB) removes the 4-sulfate group from chondroitin 4-sulfate (C4S) and dermatan sulfate and is required for their degradation. Prior work showed that ARSB immunohistochemical scores were lower in malignant prostate tissue, and were associated with higher Gleason scores and recurrence. OBJECTIVE: This study aims to confirm that ARSB immunostaining of prostate tissue obtained at the time of radical prostatectomy is prognostic for prostate cancer recurrence. METHODS: Intensity and distribution of ARSB immunostaining were digitally analyzed in a large, well-annotated, prostate cancer tissue microarray (TMA). Scores were calculated for stroma and epithelium and compared …for 191 cases, including 36 recurrences, defined as PSA > 0.2 ng/ml. RESULTS: Epithelial scores were significantly lower in the recurrences (p = 0.010), and among subgroups with age > 60, initial PSA > 6 ng/ml, or Gleason grade = 7. ARSB score did not improve the prediction of recurrence in multifactorial analysis. CONCLUSIONS: Study findings validate previous findings and provide further evidence that lower ARSB is associated with prostate cancer recurrence. Additional studies are required to assess if there are specific cutoff values that may help predict recurrence. Show more

Keywords: Sulfatase, chondroitin sulfate, versican, immunohistochemistry, glycosaminoglycans, N-acetylgalactosamine-4-sulfatase

DOI: 10.3233/CBM-170680

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 229-234, 2018

Authors: Bakhti, Seyedeh Zahra | Latifi-Navid, Saeid | Zahri, Saber | Bakhti, Fatemeh Sadat | Hajavi, Naser | Yazdanbod, Abbas

Article Type: Research Article

Abstract: BACKGROUND: Although the most extensive studies revealed the role of H. pylori VacA and CagA toxins in the development of gastric adenocarcinoma, the magnitude of this association and the correlations of vacA mosaicism and cagA status with cardia gastric adenocarcinoma (CGA) still remain controversial. OBJECTIVE: We aimed to examine the linkage of H. pylori highly cytotoxic genotypes to CGA in Iranian populations as a model. METHODS: A total of 601 Iranian patients were enrolled. Biopsies were cultured, genotyped, and anatomically and histologically classified. RESULTS: The vacA …c1 genotype, but not cagA status, showed a strong association with the risk of both CGA and non-cardia adenocarcinoma (NCGA), whether the controls were non-tumors, as those with either non-atrophic gastritis or peptic ulcerations, (the OR (95%CI) was 14.11 (4.91–40.52) and 9.59 (4.06–22.65), respectively) or those with NAG (the OR (95%CI) was 10.71 (3.49–32.82) and 8.11 (3.26–20.16), respectively). The vacA c1/cagA + genotype was significantly associated with an increased risk of NCGA, whether the controls were non-tumors or those with NAG; the adjusted risk was 4.706 (1.41–15.67) and 4.85 (1.42–16.51), respectively. CONCLUSIONS: The H. pylori vacA c1 genotype, but not cagA status, might be the first important bacterial biomarker for predicting the cardia adenocarcinoma risk in male patients aged ⩾ 55 in Iran. Show more

Keywords: Helicobacter pylori, vacA c, cagA, cardia gastric adenocarcinoma, diffuse-type gastric adenocarcinoma, intestinal-type gastric adenocarcinoma

DOI: 10.3233/CBM-170701

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 235-246, 2018

Authors: Sun, Yun-Peng | Wang, Xuan | Gao, Yong-Sheng | Zhao, Song | Bai, Yang

Article Type: Research Article

Abstract: In large autopsy series, the estimated frequency of primary tumors of the heart ranges from 0.0017% to 0.33%. Approximately 25% of primary cardiac tumors are malignant, and nearly 20% of these are sarcomas. To date, a completely feasible surgical resection remains the major treatment measure of cardiac sarcoma, especially for recurrent focal cardiac sarcoma and the recurrence of a restrictive metastasis. Although characteristically medical treatments are recommended, there is no consistent opinion for adjuvant radiotherapy and chemotherapy following an operation. Since these tumors usually undergo extensive spread by the time that the diagnosis is established, the prognosis of cardiac sarcoma remains poor. …In this report, we described a case who underwent initial cardiac tumor resection, and was confirmed to be a pleomorphic undifferentiated sarcoma based on pathological findings. However, the patient complicated with cerebral infarction and subsequent brain metastasis sarcoma after the initial surgery, which was confirmed by brain tissue pathology. During the course of therapy, the patient underwent three surgical operations and refused to accept any chemotherapy and radiotherapy intervention. To the best of our knowledge, this is the first case report describing a primary cardiac sarcoma complicated with cerebral infarction and brain metastasis. The management of primary cardiac sarcoma is also discussed. Show more

Keywords: Primary cardiac sarcoma, cerebral infarction, brain metastasis

DOI: 10.3233/CBM-170448

Citation: Cancer Biomarkers, vol. 21, no. 1, pp. 247-250, 2018