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Simulation and experimental research on micro-channel for detecting cell status in bio-artificial liver

Issue title: Papers from the 3rd International Conference on Biomedical Engineering and Technology (iCBEB 2014), 25–28 September 2014, Beijing, China

Subtitle:

Guest editors: Edward J. Ciaccio

Article type: Research Article

Authors: Wu, Changzhe | Cao, Yue | Huo, Xiaolin* | Li, Ming

Affiliations: Beijing Key Laboratory of Bioelectromagnetism, Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing, China

Correspondence: [*] Corresponding author: Xiaolin Huo, Beijing Key Laboratory of Bioelectromagnetism, Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing, China. Tel.: +86 010 82547242; Fax: +86 010 82547242; E-mail:huoxl@mail.iee.ac.cn

Keywords: Bio-artificial liver, ALT, urea, ANSYS, mixing efficiency

DOI: 10.3233/THC-150973

Journal: Technology and Health Care, vol. 23, no. s2, pp. S365-S371, 2015

Published: 2015
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Abstract

BACKGROUND: Bioartificial liver support system (BALSS) based on culturing hepatocytes is an important research field for the treatment of acute liver failure. It is necessary to monitor the state of liver cell functions during the treatment of BALSS in order to guide clinical treatment.

OBJECTIVE: To design a micro-channel chip to achieve flash mixing for timely detection of liver cell status in bioreactors and improving liver cells growth environment to ensure the efficacy of the bio-artificial liver support system.

METHODS: Alanine aminotransferase (ALT) and Urea are chosen as detection indicators to reflect the degree of liver cell injury and the detoxification function. A diamond tandem structure micro-channel is designed and optimized to achieve the efficient mixing of serum and ALT or Urea reagent.

RESULTS: The simulation and experimental results show that the diamond tandem structure micro-channel can significantly improve the mixing efficiency and meet the online detecting requirements.

CONCLUSION: The easily controllable diamond tandem structure micro-channel combines the advantages of active and passive mixer and can effectively mix the serum and ALT or Urea reagent. It lays the foundation for online monitoring of liver cells and will help to improve the viability of liver cell in the bioreactor.

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