Affiliations: [a] School of Pharmaceutical and Biological Engineering, Sichuan University, Chengdu, Sichuan, China | [b] The Second Affiliated Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China | [c] Department of Pharmacy, Zunyi Medical College, Zunyi, Guizhou, China
Correspondence:
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Corresponding author: Minghui Zhu, Chengdu University of Traditional Chinese Medicine, Chengdu 610000, Sichuan, China. Tel.: +86 028 85229807; Fax: +86 028 85250977;wg8855350@163.com
Abstract: The metabolites from human plasma and follicular
fluid in IVF-ET are related with the metabolic activity of follicular cells
and further with oocyte quality. With liquid
chromatography-time-of-flight-mass spectrometry (LC-TOF-MS) and organic
extraction from plasma and follicular fluid, a fast and efficient method was
established for detecting the metabolites of human plasma and
follicular fluid. Certain metabolites were closely related with human
physiological activities, such as hormone, amino acid, estrogen and fatty
acid,
and estrogen fatty acyllipid lipid were successfully detected in human plasma and follicular fluid. The metabolites in follicular fluid and
plasma on the day of oocyte retrieval were found to be similar. But those in
the latter and basal plasma were partially different. Methyl (3α
,5β ,7α ,12α)-3,7,12-trihydroxycholan-24-oate,
androst-4-ene-3,17-dione and tryptophan were identified and might be
potential biomarkers on oocyte quality. It demonstrates that LC-TOF-MS
combined with organic extraction could be an efficient tool to investigate
the metabolites of human plasma and follicular fluid. Based on this
study, it is expected to provide the basis for the further steps to explore
possible influential factors on oocyte quality.
Keywords: Amino acid, estrogen, follicular fluid, liquid chromatography coupled with tandem mass spectrometry (LCMS/MS), plasma
