Affiliations: [a]
Education and Research Centre
| [b]
Department of Anaesthesia and Intensive Care
| [c]
Metabolism Laboratory
| [d] Department of Vascular Surgery, St. Vincent’s Hospital, Elm Park, Dublin 4, Ireland | [e]
Dublin City University, Glasnevin, Dublin 9, Ireland
Note: [*] Dr. R. Freaney, Metabolism Laboratory, St. Vincent’s Hospital, Elm Park, Dublin 4, Ireland. Tel.: +353 1 269 45 33, ext. 4513; Fax: +353 1 260 11 86.
Abstract: Direct sensor implantation for continuous biochemical monitoring has proved disappointing, but microdialysis sampling devices can serve as a biocompatible patient-sensor interface. We propose a novel “MiniShunt” extracorporeal microdialysis sampling circuit designed to sample core blood, and report the performance of different microdialysis probe membrane types and sizes with pumped and non-pumped blood flow in anaesthetised dogs. Discrete microdialysate and plasma samples collected during glucose and lactate monitoring were analysed with a YSI analyzer and showed microdialysis efficiency (% relative recovery) in excess of 95% for both analytes using fibres of greater than 70 mm2 internal surface area. Pumped veno-venous extracorporeal blood microdialysis sampling of this type could provide a suitable interface for future multi-analyte on-line biosensor applications in critically ill patients.
