Affiliations: College of Life Science, Nanjing Normal University, Nanjing, China | Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, China | Jiangsu Province Engineering and Technology Research Center for Microbiology, College of Life Science, Nanjing Normal University, Nanjing, China | Center for Analysis and Testing, Nanjing Normal University, Nanjing, China
Note: [] Corresponding author: Qi-yin Yang, Jiangsu Province Engineering and Technology Research Center for Microbiology, College of Life Science, Nanjing Normal University, Nanjing 210046, People's Republic of China. Tel.: +86 25 85891617; Fax: +86 25 85891526; E-mail: yangqiyin@163.com.
Abstract: The interaction between bovine serum albumin (BSA) and Prodigiosin (PG) was investigated by UV-vis absorption, fluorescence, synchronous fluorescence, FT-IR and circular dichroism (CD) techniques. The data of UV-vis absorption and fluorescence spectra displayed that there existed interaction between PG and aromatic amino acid residues of BSA. The synchronous fluorescence and CD spectrum experiment both showed that the secondary structure of BSA changed with addition of PG. All these results revealed that the conformation and microenvironment of BSA were changed.
