Abstract: This paper is an empirical study investigating the effect of using a fractional Black–Scholes model for pricing Call options in comparison to the classical Black–Scholes model. We estimate the Hurst parameter by nine different methods available in software R, for a set of fifteen assets from four different industries and for various lengths of used historical data. We use implied volatility for calculation of option price with a one month time to maturity. As a main result, we provide a table of tendencies of suitability for using each of the Hurst exponent estimation methods depending on the length of underlying data set.