Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Subtitle:
Article type: Research Article
Authors: Misawa, Masakia; * | Nitta, Naotakaa | Shirasaki, Yoshioa | Hayashi, Kazuhikoa | Kosaka, Ryoa | Hyodo, Kojia | Numano, Tomokazub | Homma, Kazuhiroa | Kuribayashi, Shotaa | Fujihara, Yukoc | Hoshi, Kazutoc
Affiliations: [a] Institute for Human Science and Biomedical Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan | [b] Department of Radiological Science, Graduate School of Human Health Science, Tokyo Metropolitan University, Arakawa-ku, Tokyo, Japan | [c] Department of Cartilage and Bone Regeneration, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan
Correspondence: [*] Corresponding author: Masaki Misawa, Institute for Human Science and Biomedical Engineering, National Institute of Advanced Industrial Science and Technology (AIST), 1-2-1 Namiki, Tsukuba, Ibaraki 305-8564, Japan. Tel.: +81 29 861 7106; Fax: +81 29 861 7905; E-mail:m.misawa@aist.go.jp
Abstract: BACKGROUND: Transmission and tomographic X-ray measurements are useful in assessing bone structures, but only a few studies have examined cartilage growth because of the poor contrast in conventional X-ray imaging. OBJECTIVE: In this study, we attempted to use the linear attenuation coefficient (LAC) as a metric of tissue-engineered cartilage development, which would be useful in high-throughput screening of cartilage products. METHODS: Assuming that the LAC is related to the amount of extracellular matrix (ECM) in terms of the density and its atomic components, we measured X-ray absorption through tissue-engineered cartilage constructs. Characteristic X-ray beams from a molybdenum microfocus X-ray tube were employed to avoid beam hardening. The correlation of the LAC with mechanical properties was analyzed for verification. RESULTS: The LAC was higher for chondrocyte constructs and lower for fibroblast-dominant constructs and was consistent with the quantification of toluidine blue staining, which is a proof of ECM production. The LAC was positively correlated with the bending modulus but negatively correlated with the dynamic elastic modulus and stiffness, possibly because of the remaining scaffold. CONCLUSIONS: The LAC has the potential to be used as a metric of development of tissue-engineered cartilage. However, the calcified regions should be excluded from analysis to avoid decreasing the correlation between the LAC and the amount of ECM.
Keywords: X-ray absorptiometry, characteristic X-ray, linear attenuation coefficient, tissue-engineered cartilage, extra-cellular matrix
DOI: 10.3233/XST-150504
Journal: Journal of X-Ray Science and Technology, vol. 23, no. 4, pp. 489-502, 2015
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl