X-Ray Microscopy at Suboptical Resolution: Direct Observation of the 65-nm Periodicity in Collagen Fibrils

Article type: Research Article

Authors: Burge, R. E. | Buckley, C. J. | Foster, G. F. | Miller, A. | Wess, T.

Affiliations: Department of Physics, King's College London, Strand, London WC2R 2LS, United Kingdom | Department of Biochemistry, University of Edinburgh, George Square, Edinburgh EH8 9X1, United Kingdom

Abstract: Using a Fresnel zone plate we demonstrate for the first time the direct visualization by x-ray microscopy of suboptical regularity in a biological specimen, namely the 65-nm axial periodicity of tendon collagen. This resolution test demonstrates a resolving power of about 20λ; a resolving power of <10λ is in prospect.

DOI: 10.3233/XST-1992-3404

Journal: Journal of X-Ray Science and Technology, vol. 3, no. 4, pp. 311-314, 1992