Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Pérez, MartÍna; b; c | Lado, Gerardo M.a; c | Mato, Germána; c; d | Franco, Diego G.a; c; d | Vinciguerra, Ignacio Artolaa; b | Berisso, Mariano Gómeza; b; c; d | Pomiro, Federico J.a; b | Lipovetzky, Joséa; b; c; d; * | Marpegan, Lucianoc; *
Affiliations: [a] Centro Atómico Bariloche, Av. Bustillo 9500, S. C. de Bariloche, Rio Negro, Argentina | [b] Comisión Nacional de Energía Atómica, Argentina | [c] Instituto Balseiro, Universidad Nacional de Cuyo, Argentina | [d] Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
Correspondence: [*] Corresponding authors. José Lipovetzky and Luciano Marpegan. E-mails: lipo@cab.cnea.gov.ar (J.L.) and luciano.marpegan@cab.cnea.gov.ar (L.M.).
Abstract: An automated system for acquiring microscopic-resolution radiographic images of biological samples was developed. Mass-produced, low-cost, and easily automated components were used, such as Commercial-Off-The-Self CMOS image sensors (CIS), stepper motors, and control boards based on Arduino and RaspberryPi. System configuration, imaging protocols, and Image processing (filtering and stitching) were defined to obtain high-resolution images and for successful computational image reconstruction. Radiographic images were obtained for animal samples including the widely used animal models zebrafish (Danio rerio) and the fruit-fly (Drosophila melanogaster), as well as other small animal samples. The use of phosphotungstic acid (PTA) as a contrast agent was also studied. Radiographic images with resolutions of up to (7±0.6)μm were obtained, making this system comparable to commercial ones. This work constitutes a starting point for the development of more complex systems such as X-ray attenuation micro-tomography systems based on low-cost off-the-shelf technology. It will also bring the possibility to expand the studies that can be carried out with small animal models at many institutions (mostly those working on tight budgets), particularly those on the effects of ionizing radiation and absorption of heavy metal contaminants in animal tissues.
Keywords: Zebrafish, Drosophila, CMOS, image processing, image sensors, X-ray imaging
DOI: 10.3233/XST-230232
Journal: Journal of X-Ray Science and Technology, vol. 32, no. 2, pp. 355-367, 2024
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl