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Article type: Research Article
Authors: Li, Zhifang | Chen, Haiyu | Li, Hui | Chen, Wei R.;
Affiliations: Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education, Fuzhou, Fujian, China | Department of Cardiovascular Surgery, Fujian Provincial Hospital, Fuzhou, Fujian, China | Biophotonics Research Laboratory Department of Engineering and Physics, University of Central Oklahoma, Edmond, OK, USA
Note: [] Corresponding authors: Hui Li, Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education, Fuzhou 350007, Fujian, China. Tel.: +86 591 2286 8133; E-mail: hli@fjnu.edu.cn. Wei R. Chen, Biophotonics Research Laboratory Department of Engineering and Physics, University of Central Oklahoma, Edmond, OK 73034, USA. Tel.: +1 405 974 5147; E-mail: wchen@uco.edu
Abstract: A method combining morphological granulometry with Mie theory to determine optical scattering in biological tissues was proposed. Otsu's method was applied to binarize phase-contrast images. Binary morphological granulometry was used to estimate size density distribution of the tissue samples based on the binary phase-contrast images. Our results showed that the optical parameters associated with light scattering in tissue could be quantitatively determined by combining size density distribution with Mie theory. It was suggested that this unique method could be used to characterize biological tissues for disease diagnosis.
Keywords: Morphological granulometry, optical scattering properties, Mie theory
DOI: 10.3233/XST-140474
Journal: Journal of X-Ray Science and Technology, vol. 23, no. 1, pp. 111-118, 2015
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