Affiliations: The National Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science, Nanjing University, Nanjing 210093, China | Department of Ecology & Biodiversity, The University of Hong Kong, Pokfulam Road, Hong Kong, China
Abstract: There were 6 target DNA fragments of the three parental strains existing in the cell of GEMs (genetically engineered microorganism strain) Fhhh measured in this research by PCR (polymerase chain reaction). The determination showed that GEMs Fhhh contained all the 6 target DNA fragments, mnp1, mnp2, lip1, lip2, FLO1 and 16S rDNA, and had the molecular genetic stability. Meanwhile the PCR production of each parental strain could only had its target DNA fragments and was different from each other. It may illustrate that the technique of the inter-kingdom protoplast fusion for the construction of GEMs Fhhh through the process of intercellular gene recombination could be used as a reliable bioengineering technique to create the specific functional stain for the pollution control.
Keywords: GEMs, protoplast fusion, target gene, DNA fragment, PCR, pollution control
