Investigation of protein-styrene oxide adducts as a molecularbiomarker of human exposed to styrene

Article type: Research Article

Authors: Liu, Shu-fen | Fang, Qing-ming | Jin, Zu-liang | Rappaport, M. S.

Affiliations: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. E-mail: SFliu@mail.rcees.ac.cn | UNC at Chapel Hill. NC. USA

Abstract: Hemoglobin-styrene oxide adducts in blood has been studied as a molecular biomarker of worker exposed to styrene.Determination of protein-styrene oxide adducts in different biological samples with modified Raney-Ni procedure is described in this paper. The following biological samples have been investigated: fresh rat blood reacted with styrene oxide in vitro; rat blood reacted with styrene or styrene oxide in vivo; vein blood from workers exposed to styrene in two factories. The data showed that there was a good linear dose-response relationship between reacting dose of styrene oxide or styrene and amount of protein-styrene oxide adducts in both in vitro and in vivo experiments. For human samples, a dose-response relationship between protein adducts and styrene exposure can be found in glass fiber factory, but not in piano manufacture plant. protein adduct; styrene oxide; molecular biomarker

Keywords: , molecular biomarker, styrene oxide, protein adduct

Journal: Journal of Environmental Sciences, vol. 13, no. 4, pp. 391-397, 2001