Excessive Cu and Zn affecting on distribution of the metals and
activities of glycolytic and nitrogen incorporating key enzymes in mycelia of
ectomycorrhizal fungi Suillus bovinus
Affiliations: Department of Urban and Environmental Sciences, Peking
University, Beijing 100871, China. E-mail: yhuamg@urban.pku.edu.cn
Abstract: Concentration of copper and zinc in isolated Suillus bovinus
mycelia, used nutrient solution and 0.5 mol/L EDTA mycelia washing solution
were measured to investigate the distribution of heavy metals in mycelia growth
in excess copper or zinc nutrient solution. Treated with zinc, most of added
zinc maintained in used solution, and 9.8%/14.6% was in/on mycelia in
treatment, and in treetment 2 was 3.9%/8.0%in/on mycelia. In the copper
applications, copper stimulated in more than on mycelis, i.e., 25.9%/4.5% in/on
mycelia in treatment, and 7%/18.8% in/on mycelia while most of copper retained
in used nutrient solution. Certain amount of copper or zinc uptake by mycelia
led to pronounced influence on glycolysis and nitrogen incorporating process of
Suillus bovinus, while the tested enzymes kept constant in treatment.In crude
extracts of copper treatment 2 mycelia, activities of HK, PFK and GS were
inhibited and decrease to 63%, 48% and 38% and GlDH ncreased by 68 % of the
control, respectively. The behaviors of these tested enzymes toward sinc
corresponded in general with that towards copper. The potential protection of
Suillus bpvoninus for its host plant under excess copper or zinc threaten was
discussed.
