Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Rudenko, Lauren K.a | Wallrabe, Horsta; b | Periasamy, Ammasia; b | Siller, Karsten H.c | Svindrych, Zdenekd | Seward, Matthew E.a; e | Best, Merci N.a | Bloom, George S.a; e; f; *
Affiliations: [a] Department of Biology, University of Virginia, Charlottesville, VA, USA | [b] W.M. Keck Center for Cellular Imaging, University of Virginia, Charlottesville, VA, USA | [c] Advanced Research Computing Services, University of Virginia, Charlottesville, VA, USA | [d] Department of Biochemistry and Cell Biology, Geisel School of Medicine, Dartmouth College, Hanover, NH, USA | [e] Department of Cell Biology, University of Virginia, Charlottesville, VA, USA | [f] Department of Neuroscience, University of Virginia, Charlottesville, VA, USA
Correspondence: [*] Correspondence to: George S. Bloom, Department of Biology, University of Virginia, PO Box 400328, Charlottesville, VA 22904-4328, USA. Tel.: +1 434 243 3543; E-mail: gsb4g@virginia.edu.
Abstract: Abnormal folding and aggregation of the microtubule-associated protein, tau, is a hallmark of several neurodegenerative disorders, including Alzheimer’s disease (AD). Although normal tau is an intrinsically disordered protein, it does exhibit tertiary structure whereby the N- and C-termini are often in close proximity to each other and to the contiguous microtubule-binding repeat domains that extend C-terminally from the middle of the protein. Unfolding of this paperclip-like conformation might precede formation of toxic tau oligomers and filaments, like those found in AD brain. While there are many ways to monitor tau aggregation, methods to monitor changes in tau folding are not well established. Using full length human 2N4R tau doubly labeled with the Förster resonance energy transfer (FRET) compatible fluorescent proteins, Venus and Teal, on the N- and C-termini, respectively (Venus-Tau-Teal), intensity and lifetime FRET measurements were able to distinguish folded from unfolded tau in living cells independently of tau-tau intermolecular interactions. When expression was restricted to low levels in which tau-tau aggregation was minimized, Venus-Tau-Teal was sensitive to microtubule binding, phosphorylation, and pathogenic oligomers. Of particular interest is our finding that amyloid-β oligomers (AβOs) trigger Venus-Tau-Teal unfolding in cultured mouse neurons. We thus provide direct experimental evidence that AβOs convert normally folded tau into a conformation thought to predominate in toxic tau aggregates. This finding provides further evidence for a mechanistic connection between Aβ and tau at seminal stages of AD pathogenesis.
Keywords: Alzheimer’s disease, amyloid-β, FRET, tauopathies, tau
DOI: 10.3233/JAD-190226
Journal: Journal of Alzheimer's Disease, vol. 71, no. 4, pp. 1125-1138, 2019
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl