Affiliations: [a] Department of Biological Sciences, Kent State University at East Liverpool, East Liverpool, OH, USA | [b] Department of Neonatology, First People's Hospital, Zhenzhou, Henan, P. R. China | [c] Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY, USA
Correspondence:
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Correspondence to: Qunxing Ding, PhD, Kent State University at East Liverpool, 400 East 4th street, East Liverpool, OH 43920, USA. Tel.: 330 382 7523; Fax: 330 385 6348; E-mail: qding@kent.edu.
Note: [] Deceased in January 2010.
Abstract: It is widely accepted that oxidative stress is involved in neurodegenerative disorders such as Alzheimer's disease (AD). Ribosomal RNA (rRNA) is one of the most abundant molecules in most cells and is affected by oxidative stress in the human brain. Previous data have indicated that total rRNA levels were decreased in the brains of subjects with AD and mild cognitive impairment concomitant with an increase in rRNA oxidation. In addition, level of 5S rRNA, one of the essential components of the ribosome complex, was significantly lower in the inferior parietal lobule (IP) brain area of subjects with AD compared with control subjects. To further evaluate the alteration of 5S rRNA in neurodegenerative human brains, multiple brain regions from both AD and age-matched control subjects were used in this study, including IP, superior and middle temporal gyro, temporal pole, and cerebellum. Different molecular pools including 5S rRNA integrated into ribosome complexes, free 5S rRNA, cytoplasmic 5S rRNA, and nuclear 5S rRNA were studied. Free 5S rRNA levels were significantly decreased in the temporal pole region of AD subjects and the oxidation of ribosome-integrated and free 5S rRNA was significantly increased in multiple brain regions in AD subjects compared with controls. Moreover, a greater amount of oxidized 5S rRNA was detected in the cytoplasm and nucleus of AD subjects compared with controls. These results suggest that the increased oxidation of 5S rRNA, especially the oxidation of free 5S rRNA, may be involved in the neurodegeneration observed in AD.
