Thiophilic Interaction Chromatography (TIC) of Amyloid-β Protein Precursor

Issue title: Mini-Forum “Mitochondria in Alzheimer Disease”

Guest editors: Xiongwei Zhu

Article type: Research Article

Authors: Bourhim, Mustapha | Johnson, Tara | Oyston, Derek | Srikrishnan, Thamarapu^{; *}

Affiliations: Department of Cancer Biology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA | Department of Pathology, Case Western Reserve University, Cleveland, OH, USA

Correspondence: [*] Address for correspondence. Tel.: +1 716 845 8302; Fax: +1 716 845 4928; E-mail: thamarapu.srikrishnan@roswellpark.org.

Note: [] Communicated by Sudha Seshadri

Abstract: Neuritic plaques, one of the diagnostic characteristics of an AD, contain extracellular deposits of amyloid-β (Aβ) derived from amyloid-β protein precursor (AβPP). The objective of this study was to extract AβPP out of HEK293 cells and to purify it. Two procedures were chosen for purification of AβPP: Thiophilic Interaction Chromatography (TIC) and molecular sieving. Using Superdex 75, Superose 12, and Fractogel gel matrices, AβPP was isolated on HPLC. The chromatograms illustrate the purification of AβPP. Our method describes a new and elegant way for the extraction and purification of AβPP from HEK293 cell lines using thiophilic interaction chromatography (TIC).

DOI: 10.3233/JAD-2007-12203

Journal: Journal of Alzheimer's Disease, vol. 12, no. 2, pp. 143-149, 2007