Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Gómez-Ramos, Pilar; * | Asunción Morán, M.
Affiliations: Department of Anatomy, Histology and Neuroscience, School of Medicine, Autónoma University of Madrid, Spain
Correspondence: [*] Corresponding author: Department of Anatomy, Histology and Neuroscience, School of Medicine, Autonoma University of Madrid. c/ Arzobispo Morcillo s/n 28029, Madrid, Spain. Tel.: +34 914975360; Fax: +34 914975353; E-mail: pilar.gomez@uam.es
Abstract: Autopsied brain tissue from Alzheimer's disease patients and old non-demented controls was studied after immunocytochemistry with the 4G8 monoclonal antibody that recognizes amyloid-β peptides. Intraneuronal 4G8-positive reaction product was detected in all of the studied brains. The same brain regions in the Alzheimer's disease samples consistently showed both more immunopositive neurons and more stained reaction product per neuron than those from the non-demented brains. Ultrastructurally, the immunopositive reaction product accumulated in clusters of cytoplasmic elements that had a lipofuscin-like appearance, showed a fibrogranular content and were also closely apposed to lipid droplets located either on their periphery or within them. The most strongly 4G8-immunopositive elements had diffuse limits with their fibrogranular content free in the cytoplasm, whereas elements either without or showing only light immunoreaction had a limiting membrane. All immunopositive neurons displayed a similar pattern of clumping heterochromatin. The hypothetical neurotoxic role of intraneuronal amyloid-β peptides free in the cytoplasm is discussed as is the possible relationship between the amyloid-β peptides recognized by the 4G8 antibody and the lipid droplets which would presumably contain esterified cholesterol.
Keywords: Alzheimer disease, intraneuronal amyloid-β peptides, neurotoxicity, lipid droplet, cholesterol
DOI: 10.3233/JAD-2007-11109
Journal: Journal of Alzheimer's Disease, vol. 11, no. 1, pp. 53-59, 2007
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl