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Article type: Research Article
Authors: Li, Jialina; 1 | Li, Mingb; 1; * | Gao, Fengb | Ge, Xiaojunb
Affiliations: [a] Department of Radiology, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China | [b] Department of Radiology, Huadong Hospital Affliated to Fudan University, Shanghai, China
Correspondence: [*] Corresponding author: Ming Li, Department of Radiology, Huadong Hospital Affiliated to Fudan University, Shanghai 200433, China. Tel.: +86 02162483180; Fax: +86 02162483180; E-mail:dtcenter_hd@163.com
Note: [1] Jianlin Li and Ming Li contributed equally to this work.
Abstract: BACKGROUND: Clinical significance of microRNA (miR)-15a in human esophageal squamous cell carcinoma (ESCC) remains unclear. OBJECTIVE: To evaluate the expression level of miR-15a and to determine its potential for diagnosis and prognosis in ESCC. METHODS: Quantitative reverse transcription polymerase chain reaction was performed to examine the expression levels of miR-15a in ESCC tissues and patients' sera. The diagnostic and prognostic implications of serum miR-15a level in human ESCC were further evaluated. RESULTS: Expression levels of miR-15a in ESCC tissues and patients' sera were significantly decreased (both P< 0.001). Additionally, serum miR-15a had an optimal diagnostic cut-off point (2.29) for ESCC with sensitivity of 86.36% and specificity of 100.00%. Moreover, low serum miR-15a level more frequently occurred in ESCC patients with advanced tumor-node-metastasis, T and N stages (all P= 0.01) and poor tumor differentiation (P= 0.03). The Kaplan-Meier curve showed that low miR-15a expression was significantly associated with shorter overall survival (OS) and disease-free survival (DFS) of ESCC patients (both P< 0.001). Further multivariate analysis identified miR-15a as an independent prognostic factor for both OS and DFS (both P= 0.01). CONCLUSION: Decreased expression of miR-15a may play a crucial role in ESCC development and progression. Serum miR-15a level could be used as a potential diagnostic and prognostic marker in clinics.
Keywords: Esophageal squamous cell carcinoma, microRNA-15a, diagnosis, clinicopathological feature, prognosis
DOI: 10.3233/CBM-160667
Journal: Cancer Biomarkers, vol. 18, no. 1, pp. 11-17, 2017
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