Affiliations: [a] Department of Gastroenterology, Ruijin Hospital, Luwan Branch, Shanghai Jiaotong University School of Medicine, Shanghai, China | [b] Department of Geriatrics, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China
Correspondence:
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Corresponding authors:
Meijie Hu,
Department of Gastroenterology, Shanghai Jiaotong University School of Medicine, 149 South Chongqing Road, Shanghai 200025, China.
Tel.: +86 21 13641629810; E-mail: meijiehu0119@163.com or
Jian Li,
Department of Gastroenterology, Shanghai Jiaotong University School of Medicine, 149 South Chongqing Road, Shanghai 200025, China.
Tel.: +86 21 13301928391; E-mail: jianli59@qq.com
Abstract: Gastric cancer ranks the second highest death rate and is
the highest morbidity digestive system malignancy in China. Few reports have
elucidated the role of long non-coding RNAs (lncRNAs) in the gastric cancer
pathogenesis. The present study was aimed to identify aberrantly expressed
lncRNAs involved in the progression of gastric cancer and explored their
potential functions. A total of 1,297 lncRNAs and 2,037 mRNAs that showed
significantly differential expression level between gastric tumor samples
and matched adjacent normal tissues were identified in six pairs of samples
using microarray assay. To further explore their functions, lncRNAs were
classified into different subgroups and mRNAs were clustered into several
pathways. The expression level of 4 lncRNAs: UCA1, lincRNA-BBOX1-2, CR594506
and BC015134 were further confirmed in another cohort of 10 gastric patients
by real-time PCR assay. A coding-non-coding co-expression network revealed
that the four validated lncRNAs were correlated with twenty-six mRNAs which
gave clues about the potential roles of these lncRNAs in the process of
gastric cancer progression.
Keywords: Gastric cancer, long non-coding RNA, expression profile, UCA1
