CPT1B, a metabolic molecule, is also an independent risk factor in CN-AML

Article type: Research Article

Authors: Ling, Qing^{a; b; 1} | Mao, Shihui^{a; b; c; 1} | Pan, Jiajia^{a; b; c} | Wei, Wenwen^{a; b; c} | Qian, Yu^{a; b; c} | Li, Fenglin^{a; b; c} | Huang, Shujuan^d | Ye, Wenle^{a; b; c} | Lin, Xiangjie^{a; b; c} | Huang, Jiansong^{a; b; c} | Wang, Jinghan^{a; b; c} | Jin, Jie^{a; b; c; *}

Affiliations: [a] Department of Hematology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China | [b] Key Laboratory of Hematologic Malignancies, Diagnosis and Treatment, Hangzhou, Zhejiang, China | [c] Zhejiang University Cancer Center, Hangzhou, Zhejiang, China | [d] Department of Hematology, the First Affiliated Hospital of University of Science and Technology of China, Hefei, Anhui, China

Correspondence: [*] Corresponding author: Jie Jin, No. 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. Tel.: +86 571 87236898; Fax: +86 571 87236702; E-mail: jiej0503@zju.edu.cn.

Note: [1] Qing Ling and Shihui Mao contributed equally to this work.

Abstract: BACKGROUND: Fatty acid oxidation has been considered as an important energy source for tumorigenesis and development. Several studies have investigated the role of CPT1A, a kind of fatty acid oxidation rate-limiting enzyme, in AML. However, prognostic value and regulatory network of another subtype, CPT1B in AML remains elusive. This study aims to clarify the independent prognostic role of CPT1B in CN-AML based on clinical data and molecular level data (mRNA, miRNA and lncRNA). OBJECTIVE: The aim of this study is to investigate the prognostic value of CPT1B in AML patients. METHODS: First, we analyzed the CPT1B expression in AML cohort via the online database “GEPIA”. Subsequently, miRNA-mRNA and ceRNA networks were constructed to help predict the role of CPT1B in AML. Several molecules which showed the prognostic value and metabolic function of CPT1B were identified. Finally, the expression of CPT1B in our own cohort of 324 CN-AML patients was analyzed to clarify the results. RESULTS: It was found that CPT1B was markedly higher in AML patients compared to normal people and this upregulation was associated with the poor clinical outcome. Several molecules revealed the possible regulatory mechanism of CPT1B in AML. CONCLUSION: CPT1B is a potential prognostic factor and a therapeutic target for AML treatment.

Keywords: CPT1B, prognostic factor, differrential molecules, miRNA-mRNA network, ceRNA network

DOI: 10.3233/CBM-210043

Journal: Cancer Biomarkers, vol. 37, no. 3, pp. 133-145, 2023