Affiliations: [a] Radiation Sciences Department, Medical Research Institute, Alexandria University, Alexandria, Egypt | [b] Applied Medical Chemistry Department, Medical Research Institute, Alexandria University, Alexandria, Egypt | [c] Cancer Management and Research Department, Medical Research Institute, Alexandria University, Alexandria, Egypt | [d] Experimental and Clinical Surgery Department, Medical Research Institute, Alexandria University, Alexandria, Egypt
Correspondence:
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Corresponding author: Sanaa A. El-Benhawy, Radiation Sciences Department, Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, Alexandria 21561, Egypt. Tel.:+20 1222651127; E-mail: dr_sanaa_ali13@yahoo.com.
Abstract: BACKGROUND: Altered cadherin expression plays a vital role in tumorigenesis, angiogenesis and tumor progression. However, the function of protocadherin 17 (PCDH17) in breast cancer remains unclear. OBJECTIVE: Our target is to explore PCDH17 gene expression in breast carcinoma tissues and its relation to serum angiopoietin-2 (Ang-2), carbonic anhydrase IX (CAIX) and % of circulating CD34+ cells in breast cancer patients (BCPs). METHODS: This study included Fifty female BCPs and 50 healthy females as control group. Cancerous and neighboring normal breast tissues were collected from BCPs as well as blood samples at diagnosis. PCDH17 gene expression was evaluated by RT-PCR. Serum Ang-2, CAIX levels were measured by ELISA and % CD34+ cells were assessed by flow cytometry. RESULTS: PCDH17 was downregulated in cancerous breast tissues and its repression was significantly correlated with advanced stage and larger tumor size. Low PCDH17 was significantly correlated with serum Ang-2, % CD34+ cells and serum CAIX levels. Serum CAIX, Ang-2 and % CD34+ cells levels were highly elevated in BCPs and significantly correlated with clinical stage. CONCLUSIONS: PCDH17 downregulation correlated significantly with increased angiogenic and hypoxia biomarkers. These results explore the role of PCDH17 as a tumor suppressor gene inhibiting tumor growth and proliferation.
Keywords: Breast cancer, PCDH17, Ang-2, CD34, serum CAIX
