Up-regulation of circ_0000353 impedes the proliferation and metastasis of non-small cell lung cancer cells via adsorbing miR-411-5p and increasing forkhead box O1
Affiliations: [a] Department of Radiation Oncology, Shanghai Cancer Center, Fudan University, Shanghai, China | [b] Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China | [c] Institute of Thoracic Oncology Fudan University, Shanghai, China | [d] Department of Chest Surgery, Minhang Hospital, Fudan University, Shanghai, China | [e] Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Affiliated to Tongji University, Shanghai, China
Correspondence:
[*]
Corresponding authors: Wei-Hua Wang, Department of Chest Surgery, Minhang Hospital, Fudan University, 170 Xinsong Road, Minhang, Shanghai, China. E-mail: shangzhangwu2@163.com. Min-wei Bao, Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Affiliated to Tongji University, Zheng Min Road No. 507, Shanghai, China. E-mail: ganli0603@163.com.
Note: [1] Wei-xin Zhao and Yan-lei Tang contribute equally to this work.
Abstract: BACKGROUND: Non-small cell lung cancer (NSCLC) is the most common malignant tumor worldwide. This work focuses on investigating the role of circ_0000353 in NSCLC and its potential mechanism of action. METHODS: The expression levels of circ_0000353 and miR-411-5p in NSCLC and their matched normal lung tissues were detected by real-time PCR (RT-PCR). The correlation between the circ_0000353 expression and the clinicopathological parameters of NSCLC patients was also analyzed. CCK-8, BrdU and colony formation assays were adopted to detect the role of circ_0000353 in the proliferation of NSCLC cells. The metastasis of NSCLC cells was measured by Transwell assay. The dual-luciferase reporter gene assay was used to confirm the targeting relationship between circ_0000353 and miR-411-5p. The expression level of FOXO1 was detected by western blot. RESULTS: Circ_0000353 was significantly down-regulated in NSCLC tissues and cell lines, and the decreased expression was significantly linked to the increased clinical stage, larger tumor volume, and metastasis. The circ_0000353 over-expression restrained the proliferation, migration, and invasion of NSCLC cells in vitro. Additionally, up-regulation of miR-411-5p was observed in NSCLC tissues and cell lines, and luciferase assay and RT-PCR assay showed that circ_0000353 over-expression could target miR-411-5p and suppress its expression. Further studies confirmed that circ_0000353 and miR-411-5p modulated the FOXO1 expression. CONCLUSION: Circ_0000353 repressed the proliferation, migration, and invasion of NSCLC cells via inhibition of miR-411-5p and up-regulation of FOXO1.
