Affiliations: [a] Gynecology and Obstetrics Department of the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong, China | [b] Gynecology and Obstetrics Department, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China
Correspondence:
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Corresponding author: Lin Lu, Gynecology and Obstetrics Department, Nanfang Hospital, Southern Medical University, No 1838 North of Guangzhou Avenue Baiyun District of Guangzhou, Guangzhou 510515, Guangdong, China. Tel.: +86 13711138184; E-mail: lulinlulin@21cn.com.
Abstract: The SerpinE2 pathway is evolutionarily conserved and plays an important role in tumorigenesis. SerpinE2 (a small ubiquitin-related modifier), like ubiquitin, conjugates SerpinE2 proteins onto lysine residues of target proteins. SerpinE2 over-expression has been found in several tumors. Here, we detected the level of SerpinE2 in 72 samples of EC tissue using immunohistochemistry to assess the role of SerpinE2 in EC prognosis. Meanwhile, we knocked down SerpinE2 by siRNA in the HTB-111 and Ishikawa EC cell lines and analyzed the viability and mobility change using an MTT assay, an annexin V/PI apoptosis assay, a wound scratch test and a transwell assay. A Kaplan-Meier analysis indicated a negative correlation between the level of SerpinE2 and the EC prognosis. Silencing SerpinE2 induced cell apoptosis and reduced the migration ability. Our data suggest SerpinE2 works as an oncogene in EC.
