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Article type: Research Article
Authors: Huang, Kai* | Chen, Jun | Yang, Mo-Song | Tang, Yu-Jun | Pan, Feng
Affiliations: Department of Orthopedics, Shanghai Zhabei District Central Hospital, Shanghai, China
Correspondence: [*] Corresponding author: Kai Huang, Department of Orthopedics, Shanghai Zhabei District Central Hospital, Zhonghuaxin Road Zhabei District, Shanghai 200070, China. E-mail:13817784210@163.com
Abstract: Chondrosarcomas are malignant cartilage-forming tumors from low-grade to high-grade aggressive tumors characterized by metastasis. Cisplatin is an effective DNA-damaging anti-tumor agent for the treatment against a wide variety of solid tumors. However, chondrosarcomas are notorious for their resistance to conventional chemo- and radio- therapies. In this study, we report miR-23b acts as a tumor suppressor in chondrosarcoma. The expressions of miR-23b are down-regulated in chondrosarcoma patient samples and cell lines compared with adjacent normal tissues and human primary chondrocytes. In addition, overexpression of miR-23b suppresses chondrosarcoma cell proliferation. By comparison of the cisplatin resistant chondrosarcoma cells and parental cells, we observed miR-23b was significantly down regulated in cisplatin resistant cells. Moreover, we demonstrate here Src kinase is a direct target of miR-23b in chondrosarcoma cells. Overexpression of miR-23b suppresses Src-Akt pathway, leading to the sensitization of cisplatin resistant chondrosarcoma cells to cisplatin. This chemo-sensitivity effect by the miR-23b-mediated inhibition of Src-Akt pathway is verified with the restoration of Src kinase in miR-23b-overespressing chondrosarcoma cells, resulting in the acquirement of resistance to cisplatin. In summary, our study reveals a novel role of miR-23b in cisplatin resistance in chondrosarcoma and will contribute to the development of the microRNA-targeted anti-cancer therapeutics.
Keywords: microRNA-23b, chondrosarcoma cells, cancer therapeutics, Src-Akt pathway
DOI: 10.3233/CBM-160102
Journal: Cancer Biomarkers, vol. 18, no. 3, pp. 231-239, 2017
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