Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Trojani, Alessandraa; * | Di Camillo, Barbarab | Tedeschi, Alessandraa | Lodola, Milenaa | Montesano, Simonac | Ricci, Francescaa | Vismara, Eleonoraa | Greco, Antoninoa | Veronese, Silviod | Orlacchio, Aldoc | Martino, Sabatac | Colombo, Chiaraa | Mura, Mariangelae | Nichelatti, Michelef | Colosimo, Annad | Scarpati, Barbarag | Montillo, Marcoa | Morra, Enricaa
Affiliations: [a] Division of Hematology, Niguarda Hospital, Milan, Italy | [b] Department of Information~Engineering, University of Padova, Padova, Italy | [c] Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy | [d] Department of Laboratory Medicine, Molecular Pathology Unit, Niguarda Hospital, Milan, Italy | [e] Department of Laboratory medicine, Pathological Anatomy and Cytogenetics, Niguarda Hospital, Milan, Italy | [f] Service of Biostatistics, Niguarda Hospital, Milan, Italy | [g] Department of Transfusion Medicine and Division of Hematology, Niguarda Hospital, Milan, Italy
Correspondence: [*] Corresponding author: Alessandra Trojani, Department of Oncology, Division of Hematology, Niguarda Hospital, Piazza Ospedale Maggiore 3, 20162, Milan, Italy. Tel.: +39 02 64442711; Fax: +39 02 64447598; E-mail: alessandra.trojani@ospedaleniguarda.it.
Abstract: Background:Several studies demonstrated IGVH mutational status and ZAP70 expression as the most relevant prognostic markers in Chronic Lymphocytic Leukemia (CLL), suggesting the separation of two patient subgroups: with good mutated ZAP70 negative (MTZAP70-) and poor unmutated ZAP70 positive (UMZAP70+) prognosis. Design and methods:We determined the gene expression of B cells in 112 CLL patients divided into three classes: class 1 with MTZAP70-, class 2 with UMZAP70+, and class 3 included both UMZAP70- and MTZAP70+. Results:We found LPL, AGPAT2, MBOAT1, CHPT1, AGPAT4, PLD1 genes encoding enzymes involved in lipid metabolism overexpressed in UMZAP70+. In addition, this study identified ARSD, a gene belonging to the sphingolipid metabolism, as a new gene significantly overexpressed in UMZAP70+ compared to MTZAP70-. Western blots confirmed that ARSD protein levels were significantly different between the 3 classes of patients and normal controls. Statistical analysis identified a significant correlation between ARSD and IGVH; however, both ARSD protein level and IGVH were independently associated with the need for therapy of CLL patients. Conclusions:ARSD is a novel prognostic factor as the time to start therapy is shorter in patients with high levels of ARSD protein and sphingolipid metabolism could represent a new biological mechanism in CLL.
Keywords: CLL, microarray, IGVH, ARSD
DOI: 10.3233/CBM-2012-0259
Journal: Cancer Biomarkers, vol. 11, no. 1, pp. 15-28, 2012
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl