Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Petronilli, Valeria | Miotto, Giovanni | Canton, Marcella | Colonna, Raffaele; | Bernardi, Paolo; | Di Lisa, Fabio; ;
Affiliations: Centro per lo Studio delle Biomembrane, CNR, Università di Padova, Via G. Colombo 3, 35121 Padova, Italy | Dipartimento di Chimica Biologica, Università di Padova, Via G. Colombo 3, 35121 Padova, Italy | Dipartimento di Scienze Biomediche, Università di Padova, Via G. Colombo 3, 35121 Padova, Italy
Note: [] Correspondence to: Prof. Fabio Di Lisa, Dipartimento di Chimica Biologica, Via G. Colombo 3, 35121 Padova, Italy. Fax: +39 049 8073310; E‐mail: dilisa@civ.bio.unipd.it.
Abstract: The involvement of mitochondrial permeability transition pore (MTP) in cellular processes is generally investigated by indirect means, such as changes in mitochondrial membrane potential or pharmacological inhibition. However, such effects could not be related univocally to MTP. In addition, source of errors could be represented by the increased retention of membrane potential probes induced by cyclosporin A (CsA) and the interactions between fluorescent probes. We developed a direct technique for monitoring MTP. Cells were co‐loaded with calcein‐AM and CoCl_2, resulting in the quenching of the cytosolic signal without affecting the mitochondrial fluorescence. MTP inducers caused a rapid decrease in mitochondrial calcein fluorescence which, however, was not completely prevented by CsA. Besides the large and rapid efflux of calcein induced by MTP agonists, we also observed a constant and spontaneous decrease of mitochondrial calcein which was completely prevented by CsA. Thus, MTP likely fluctuates between open and closed states in intact cells.
Keywords: Mitochondria, calcium, calcein, permeability transition pore, cyclosporin
Journal: Biofactors, vol. 8, no. 3-4, pp. 263-272, 1998
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl