Affiliations: Department of Embryology, Reproductive Medicine
Center, Royan Institute, Tehran, Iran | Department of Anatomy, Faculty of Medicine,
Baqiatallah (a.s.) University of Medical Sciences, Tehran, Arak, Iran | Department of Biology, Faculty of Basic Science, Arak
University, Arak, Iran | Department of Anatomy, Faculty of Medicine, Tarbiat
Modarres University, Tehran, Iran
Note: [] Address for correspondence: H. Eimani, Department of Embryology,
Reproductive Medicine Center, Royan Institute, P.O. Box: 19395-4644, Tehran,
Iran. Tel.: +98 21 22305236; Fax: +98 21 22306481; E-mail: Eimanih@yahoo.com
Abstract: The aim of this study was to investigate the growth and survival
rate of preantral follicles isolated from vitrified ovarian tissue by Cryotop
and conventional methods. The ovaries of 14-day-old mice were separated and
divided into four groups as following: Cryotop group, vitrified by Cryotop; CV
(Conventional; CV) group, vitrified by conventional straw; toxicity test group
and control group. After warming the vitrified ovaries, isolated preantral
follicles from four groups were cultured for 4 days to compare survival rate
and follicular growth between above-mentioned groups. Survival rate (97.3%)
in toxicity test group alike the control group (98.7%) were significantly
higher (P < 0.05) than the Cryotop (92.7%) and CV (47.7%) groups.
Increase in follicle diameters after 4 days in Cryotop and CV groups was
significantly lower (P < 0.05) than the control and toxicity test groups,
but growth and survival rate of follicles in Cryotop group was significantly
higher (P < 0.05) than the CV group. These results demonstrated that ovarian
tissue vitrification by Cryotop highly preserves the viability rate of
preantral follicles.
