Affiliations: Korea Food Research Institute, San 46-1, Bundang-gu,
Sungnam-si Kyunggi-do, 463-746, Korea | Graduate School of East-West Medical Science, Kyung
Hee University, Yongin-si, Kyunggi-do, 499-701, Korea
Note: [] Address for correspondence: Inwook Choi, Ph.D., Korea Food
Research Institute, San 46-1, Bundang-gu, Sungnam-si, Kyunggi-do, 463-746,
Korea. Tel.: +82 31 780 9097; Fax: +82 31 709 9876; E-mail: choiw@kfri.re.kr
Abstract: Polyphenolic compounds were examined for their effects on
suppressing adipocyte differentiation in 3T3-L1 cells. Most polyphenolic
compounds inhibited adipocyte development from 3T3-L1 cells to some extent.
Among them, rutin was the most effective in suppressing adipocyte
differentiation in a dosage dependant manner. Activity of glycerol-3-phosphate
dehydrogenase (GPDH), which has a central position in lipogenesis in adipose
cells, was also decreased by rutin addition at the induction stage. RT-PCR
results demonstrated that mRNA expression of adipogenic transcription factors
such as peroxisome proliferator-activated receptor-γ
(PPARγ) and CCAAT/enhancer binding
protein-α (C/EBPα) in 3T3-L1
cells were remarkably down regulated by rutin treatment. For further
investigation on anti-adipogenic activities of rutin, it was orally
administered (25 and 50 mg/kg b.w/daily) with high-fat diet (64.4% of total
calories as fat) to C57BL/6 mice. Body weight gains were less in high-fat diet
+ rutin fed groups (HFR) than high-fat diet alone fed group
(HF) after 4 weeks. Total cholesterol contents in blood were significantly
lower in HFR groups. When mRNA expressions of PPARγ and
C/EBPα in hepatocytes were compared between the control
HF and HFR groups, their expressions in hepatocytes of HFR groups were
significantly suppressed. These results indicate that rutin inhibits adipogenic
development in pre-adipocytes and hepatocytes by down regulating expressions of
key adipogenic transcription factors.
