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Issue title: The Proceedings of the 3rd International Conference on Food Factors (ICoFF 03)
Article type: Research Article
Authors: Kimura, Toshiyuki | Nakagawa, Kiyotaka | Saito, Yuko | Yamagishi, Kenji | Suzuki, Masahiro | Yamaki, Kohji | Shinmoto, Hiroshi | Miyazawa, Teruo
Affiliations: National Agricultural Research Center for Tohoku Region, Fukushima 960-2156, Japan | Food and Biodynamic Chemistry Laboratory, Graduate School of Life Science and Agriculture, Tohoku University, Sendai 981-8555, Japan | Fukushima Technology Center Aizuwakamatsu Technical Support Center, Aizuwakamatsu 965-0006, Japan | National Food Research Institute, Tsukuba 305-8642, Japan
Note: [] Address for correspondence: Toshiyuki Kimura, Laboratory of Post-harvest Crop Management, National Agricultural Research Center for Tohoku Region, 50 Harajukuminami, Arai, Fukushima-city, Fukushima, 960-2156, Japan. Tel.: +81 24 593 6178; Fax: +81 24 593 2155; E-mail: kmr@affrc.go.jp
Abstract: A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from extract of mulberry leaves on TSK gel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During post column detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.
Keywords: 1-Deoxynojirimycin, evaporative light scattering detector, mulberry leaves, morus alba
Journal: BioFactors, vol. 22, no. 1-4, pp. 341-345, 2004
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