Affiliations: [a] Department of Surgical Oncology, Affiliated Hospital of Hebei United University, Tangshan, Hebei, China | [b] Department of Surgical Oncology, Tangshan People's Hospital, Tangshan, Hebei, China
Correspondence:
[*]
Corresponding author: Wanning Hu, Department of Surgical Oncology, Tangshan People's Hospital, Tangshan, Hebei, China. Fax: +86 315 3725861;tsyjydoctor55@163.com
Abstract:
BACKGROUND: Epidemiological and experimental carcinogenesis studies
provide evidence that components of garlic have anticancer activity.
OBJECTIVE: In this study, the apoptotic effects of Garlic-derived
compound S-allylmercaptocysteine (SAMC) were investigated in 8305C human
anaplastic thyroid carcinoma cells.
METHODS: The cell line 8305C
(HPACC) were treated with SAMC and the MTT assay, flow cytometry (FCM),
electron microscope method were used to test cell cycle, inhibitory rate and
morphologic changes respectively.
RESULTS: HPACC-8305C cells were
suppressed after exposure to SAMC of 0.02 mg/ml, 0.06 mg/ml, and 0.1 mg/ml
for 48 h. Compared with the control, the difference was significant
(P< 0.05). SAMC could induce apoptosis of the cells in a dose-dependent and
non-linear manner and increase the proportion of cells in the G2/M phase.
Compared with the control, the difference was significant in terms of the
percentage of cells in the G2/M phase (P< 0.05). After exposure to SAMC at
0.02 mg/ml for 24 hours, HPACC-8305C cells showed typical morphologic
change.
CONCLUSIONS: SAMC inhibits the growth of HPACC-8305C cells
by induction of apoptotic cell death and inhibit telomerase activity, which
appears to account for its anti-cancer activity.
