Affiliations: [a] Medical Research Centre, Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan | [b] Department of Operative Dentistry, Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan | [c] Department of Science of Dental Materials, Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan | [d] Department of Restorative Dental Sciences, College of Dentistry, King Faisal University, Al Ahsa, Kingdom of Saudi Arabia
Correspondence:
[*]
Corresponding author: Shahid Ali, Medical Research Centre, Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan. E-mail:shahid.ali@lumhs.edu.pk
Abstract: BACKGROUND: The development of resin dental composite restorative
materials (RDCRM) with antimicrobial activity may prevent the problem of
secondary caries and consequently prolong longevity of RDCRM.
OBJECTIVE: This study explored antibacterial activity and
hydrolytic stability of RDCRM containing chitosan.
METHODS: The antibacterial activity of microhybrid and flowable
RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against
Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact
methods. Hydrolytic stability of experimental RDCRM was evaluated using
gravimetric analysis.
RESULTS: The control and experimental flowable and microhybrid
RDCRM exhibited no growth inhibition zone in the lawn growth of
Lactobacilli casei. The direct contact test revealed that colony forming unit count of
Lactobacilli casei was comparable among the experimental and control RDCRM. The water sorption
and solubility values of control as well as experimental flowable and
microhybrid RDCRM were found to be within the ISO accepted range. There was
statistically no significant difference in water sorption and solubility of
different experimental RDCRM groups.
CONCLUSIONS: The one percentage of CS into experimental RDCRM have
no antibacterial activity against Lactobacilli casei. The hydrolytic stability of RDCRM
containing CS was acceptable.
Keywords: Resin dental composites, chitosan, antibacterial activity, water sorption, water solubility
