Affiliations: [a] Department of Biotechnology, National Formosa University, Yunlin, Taiwan | [b] Division of Gastroenterology and Hepatology, Department of Internal Medicine, Wan Fang Hospital, Taipei, Taiwan | [c] Graduate Institute of Medical Science, Taipei Medical University, Taipei, Taiwan | [d] Department of Biochemistry and Molecular Cell Biology, School of Medicine, Taipei Medical University, Taipei, Taiwan
Correspondence:
[*]
Corresponding author: Fu-Der Mai, Graduate Institute of Medical Science/Department of Biochemistry and Molecular Cell Biology, School of Medicine, Taipei Medical University, Taipei 11013, Taiwan. E-mail:fordmai@tmu.edu.tw
Abstract: Jelly fig (Ficus awkeotsang) achenes have been utilized to prepare a
traditional drink in Taiwan. Herein, we evaluated the effect of water
extract from jelly fig seed residues (WERJFA) on cancer cells. WERJFA could
inhibit the growth of human colorectal cancer cells, COLO205 and HT29 in
both dose- and time-dependent manners. The flow cytometric analysis with
propidium iodide (PI) showed that WERJFA primarily arrested COLO205 and HT29
cells at the G2/M phase of cell cycle as the concentration reached to at
least 0.5 mg/ml. WERJFA induced apoptosis of these two cell lines, as
evidenced by annexin V-FITC/PI and 4', 6-diamidino-2-phenylindole (DAPI)
staining using flow cytometry and confocal microscopy, respectively.
Reactive oxygen species (ROS) production and the loss of mitochondrial
membrane potential in WERJFA-treated cells were detected by flow cytometry
with H2DCF-DA and 5,5', 6,6'-Tetrachloro-1, 1',
3,3'-tetraethylbenzimidazolocarbocyanine iodide (JC-1). Our
results showed that WERJFA exerted anti-proliferative and apoptotic effects
on colorectal cancer cells. WERJFA arrested cell cycle, and caused apoptotic
death in these cancer cells possibly via mitochondrial pathway involved with
exceeding ROS level.
