Affiliations: Jiangsu Key Laboratory Biofunctional Materials, Nanjing Normal University, Nanjing, P. R. China
Note: [] Corresponding author: Jia-Hong Zhou, Jiangsu Key Laboratory Biofunctional Materials, Nanjing Normal University, Nanjing 210097, P. R. China. Tel./Fax: +86 25 85898170; E-mail: zhoujiahong@njnu.edu.cn.
Abstract: The interaction of Elsinochrome A (EA) with calf thymus DNA (CT-DNA) has been investigated by UV-vis spectra and fluorescence spectra. The results show that EA can bind with CT-DNA and binding sites are destroyed after irradiation by visible light, which indicates that EA is a promising candidate for photodynamic therapy. In addition, the binding mechanism is studied using fluorescence quenching test and ethidium bromide (EB) replace assay experiments. The results suggest that EA and CT-DNA are binding with a strong force and the major binding mode of EA with DNA could be the electrostatic binding.
Keywords: Elsinochrome A, fluorescence quenching, photodamage, thermodynamics parameter
DOI: 10.3233/SPE-2011-0542
Journal: Spectroscopy, vol. 26, no. 4-5, pp. 289-296, 2011
