Infrared microspectroscopic imaging as a probing tool to fast distinguish chemical compositions in calcified deposits of prostatic calculi and calcific tendonitis
Affiliations: Department of Biotechnology, Yuanpei University, Hsin-Chu, Taiwan, China | Department of Radiology, Taipei Veterans General Hospital, and School of Medicine, National Yang-Ming University, Taipei, Taiwan, China | Division of Urology, Department of Surgery, Lotung Pohai Hospital, Lotung, Ilan, Taiwan, China
Note: [] Corresponding author: Prof. Shan-Yang Lin, Ph.D., Department of Biotechnology, Yuanpei University, Hsin Chu, Taiwan, China. Tel.: +886 03 5381183~8157; Fax: +886 03 6102328; E-mail: sylin@mail.ypu.edu.tw.
Abstract: The specificity and homogeneity of the real compositional components within the calcified deposits of prostatic calculi and calcific tendonitis were investigated using Fourier transform infrared (FT-IR) microspectroscopy with or without automatic imaging system. The second-derivative analysis was also applied to differentiate the overlapping components of individual spectra for the calcified samples. The FT-IR microscopic imaging results of present study indicate that the complicated components such as protein, type B or type A carbonated apatite, brushite and calcium oxalate monohydrate were contained in the calcified tissue of prostatic tissue, but the protein, type A and type B carbonated apatites were mainly included in the calcific tendonitis. However, the traditional manually single-point FT-IR spectral result only reveals a little component contained in the calcified tissues, leading to an inaccurate diagnose of the complicated components in the calcified mixture.
