Affiliations: Division of Spectroscopy of Functional Materials, Institute of Physics, Faculty of Physics and Astronomy, University of Zielona Góra, Zielona Góra, Poland | Department of Biophysics, School of Pharmacy, Medical University of Silesia, Sosnowiec, Poland | Department of Pharmaceutical Chemistry, School of Pharmacy, Medical University of Silesia, Sosnowiec, Poland
Note: [] Corresponding author: Dr. Lidia Najder-Kozdrowska, Division of Spectroscopy of Functional Materials, Institute of Physics, Faculty of Physics and Astronomy, University of Zielona Góra, Szafrana 4a, 65-516 Zielona Góra, Poland. Tel.: +48 68 328 29 05; E-mail: lkozdrowska@wp.pl.
Abstract: This work comprises the study of DOPA–melanin complexes with kanamycin and copper(II) ions made by electron paramagnetic resonance EPR spectroscopy. The high concentration of paramagnetic centers in melanin makes the use of EPR spectroscopy possible. The unpaired electron localized on the oxygen atom in indol-5,6-quinone groups is the paramagnetic centers in this polymer. The aim of this work was the analysis of EPR parameters of recorded spectra. For researches were prepared melanin complexes which differed in complexing order of drug and Cu(II) ions, and in concentration of CuCl2 solutions used during synthesis. The introduction of drugs and metal ions into melanin causes changes in EPR parameters of recorded spectra. Kanamycin causes the increase and copper(II) ions the decrease of concentrations of paramagnetic centres in melanin. Shape of EPR signal of DOPA–melanin was analyzed by fitting method. Results indicate that EPR line of DOPA–melanin is superposition of Gaussian and Lorentzian function.
Keywords: EPR, DOPA–melanin, kanamycin, Cu(II)
DOI: 10.3233/SPE-2011-0504
Journal: Spectroscopy, vol. 25, no. 3-4, pp. 197-205, 2011
