Effect of different agents onto multidrug resistant tumor cells revealed by fluorescence correlation spectroscopy

Issue title: From Molecule to Tissue: XII European Conference on the Spectroscopy of Biological Molecules, Bobigny, France, 1–6 September 2007, Part 2 of 2

Article type: Research Article

Authors: Boutin, Céline | Roche, Yann | Millot, Christine | Deturche, Régis | Plain, Jérôme | Jeannesson, Pierre | Manfait, Michel | Royer, Pascal | Millot, Jean-Marc | Jaffiol, Rodolphe^;

Affiliations: LNIO (Laboratoire de Nanotechnologie et d'Instrumentation Optique), Institut Charles Delaunay, FRE CNRS 2848, Université de Technologie de Troyes, 10 010 Troyes cedex, France | Unité MéDIAN (Médicament, Dynamique Intracellulaire, Architecture Nucléaire), UMR CNRS 6142, Faculté de Pharmacie, Université de Reims Champagne-Ardennes, 51 096 Reims cedex, France

Note: [] Corresponding author: R. Jaffiol, LNIO (Laboratoire de Nanotechnologie et d'Instrumentation Optique), Institut Charles Delaunay, FRE CNRS 2848, Université de Technologie de Troyes, 12 rue Marie Curie, BP2060, 10 010 Troyes cedex, France. Tel.: +33 3 25718527; Fax: +33 3 25718456; E-mail: rodolphe.jaffiol@utt.fr.

Abstract: Fluorescence correlation spectroscopy (FCS) has been used to analyze the plasma membrane fluidity and heterogeneity of multidrug resistant cells. At the single cell level, the effects of different membrane agents present in the extra-cellular medium have been explored. Firstly, we reveal a modification of plasma membrane heterogeneities according to the addition of a fluidity modulator, benzyl alcohol. On the other hand, revertants such as verapamil and cyclosporin-A appear to act more specifically on the slow diffusion sites, such as lipids microdomains.

Keywords: Fluorescence correlation spectroscopy, membrane dynamic, multidrug resistant (MDR) cells

DOI: 10.3233/SPE-2008-0348

Journal: Spectroscopy, vol. 22, no. 4, pp. 261-265, 2008