Affiliations: CNISM–INFM–CNR and Department of Physics and Mathematics, University of Insubria at Como, Como, Italy | Faculty of Pharmacy, University of Iceland, Hagi, Reykjavik, Iceland | School of Pharmacy, University of Oslo, Oslo, Norway
Note: [] Corresponding author: Luca Nardo, CNISM–INFM–CNR and Department of Physics and Mathematics, University of Insubria at Como, Via Valleggio, 11-22100 Como, Italy. Tel.: +39 031 2386272; Fax: +39 031 2386119; E-mail: luca.nardo@uninsubria.it.
Abstract: Curcumin is the main constituent of curry. In its ground state it shows chemo-preventive, chemo-therapeutic and anti-inflammatory effects. For its immunostimulating action it has been considered for the development of drugs suitable for treating AIDS and cystic fibrosis. Further biological action is induced in curcumin by photoactivation: in suitable environmental conditions electronically excited curcumin can act as a singlet oxygen generator. Moreover, cytotoxicity is enhanced by light exposure and antibacterial effects are photosensitized. This work is aimed to understand the photobiological action of curcumin by elucidating the deactivation mechanisms of its first excited singlet state. In particular we find evidence of the role of tautomerization in the excited state by measuring fluorescence lifetimes and quantum yields for such compound dissolved in solvents of different polarity and H-bonding capability. Degradation quantum yield and singlet oxygen generation efficiency were also measured in acetonitrile and methanol. The results emphasize the strong dependence of the deactivation processes from the environment. The deactivation phenomenology can be fully explained by postulating intramolecular proton transfer in the cis enol conformer to be the leading non-radiative deactivation pathway.
Keywords: Curcumin, photosensitizer, excited state intramolecular proton transfer
DOI: 10.3233/SPE-2008-0335
Journal: Spectroscopy, vol. 22, no. 2-3, pp. 187-198, 2008
