Affiliations: Analysis & Testing Center, Nanjing Normal University, 210097 Nanjing, P.R. China | Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100101, P.R. China
Note: [] Corresponding author: Jiahong Zhou, Associate Professor, Analysis & Detecting Center, Nanjing Normal University, 122 Ninghai Road, Nanjing, Jiangsu 210097, P.R. China. Fax: +86 25 8359 8359; E-mail: zhoujiahong@njnu.edu.cn.
Abstract: Laser Raman spectroscopy was used to detect the nature of the structural change in lysozyme sensitized by hypocrellin A (HA) at the molecular level. The results indicated that the orderly structure of lysozyme has been damaged by the active oxygen (1O2, O2−· and $\bfcdot$OH, etc.) generated by HA, and cause the changes in H-bonds system of the main chain and the side chain of lysozyme.
Keywords: Hypocrellin A, lysozyme, photosensitive damage, Raman spectroscopy, protein conformation
Journal: Spectroscopy, vol. 20, no. 5-6, pp. 269-273, 2006
