A new method for studying platelets, based upon the low-angle light scattering technique. 3. Aggregation hypersensitivity of platelets (ADP agonist) and search for corrective agents
Affiliations: I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St-Petersburg, Russia | Research Institute of Hygiene, Occupational Pathology and Human Ecology, St-Petersburg, Russia | I.P. Pavlov Medical University, St-Petersburg, Russia | School of Allied Health Sciences, De Montfort University, Leicester, UK
Note: [] Corresponding author: R.O. Jenkins, School of Allied Health Sciences, De Montfort University, Leicester, LE1 9BH, UK. Tel.: +44 116 2506306; E-mail: roj@dmu.ac.uk.
Abstract: A new method for studying platelets based on low-angle light scattering has previously revealed that platelets taken from pregnant women with preeclampsia are hypersensitive to ADP, with aggregation developing at concentrations of 7–15 nmol l−1. The method has been applied to further studies in experimental toxicology and clinical pathology. Toxicological experiments with fluoroacetate (FA), an inhibitor of TCA cycle, showed that the platelet hypersensitivity could also be caused by energy depletion. In modeling experiments, the low-angle light scattering method was applied to assessment of potential corrective agents of the pathological states related to hypersensitivity of platelets. Sodium glutamate (SG) was shown to be a potent antiaggregant in vitro, and subsequent in vivo studies demonstrated that SG can apparently serve as anaplerotic agent and normalize the platelet status of rats intoxicated with FA. Donators of nitric oxide (NO), such as isosorbide-5′-dinitrate, can also normalize in vitro the hypersensitive status of platelets taken from the patients with preeclampsia.
