Determination of 1,5‐anhydro‐glucitol–carrier protein conjugates by matrix‐assisted laser desorption/ionization tof mass spectrometry and antibody formation

Article type: Research Article

Authors: Xuan, Li‐Jiang | Tanaka, Hiroyuki | Morimoto, Satoshi | Shoyama, Yukihiro^; | Akanuma, Hiroshi | Muraoka, Keiji

Affiliations: Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi 3‐1‐1, Higashiku, Fukuoka 812‐8582, Japan | Department of Chemistry, College of Arts and Sciences, University of Tokyo, Meguroku, Tokyo 153, Japan | Higashichikushi Junior College, 5‐1‐1 Shimoitozu Kokurakita‐ku, Kitakyushu, Japan

Note: [] Corresponding author: Yukihiro Shoyama. Tel./Fax: +81 92 642 6580; E‐mail: shoyama@shoyaku.phar.kyushu‐u.ac.jp.

Abstract: In order to prepare the monoclonal antibody against 1,5‐anhydro‐glucitol (1), it was conjugated with bovine serum albumin (BSA), human serum albumin (HSA) or chicken lysozyme (CL) using succinate or β‐alanine succinate as a spacer to produce individual antigen conjugates. The number of hapten contained in each antigen conjugate was determined by matrix‐assisted laser desorption/ionization tof mass (MALDI‐tof‐MS) spectrometry. The formation of monoclonal antibody (MAb) was also discussed.

Journal: Spectroscopy, vol. 14, no. 3, pp. 85-92, 2000