Affiliations: Laboratory of Macromolecular and Organic Chemistry, Department of Chemical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands | Laboratory of Instrumental Analysis, Department of Chemical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands | Traditional Medicinal Institute, Peruvian Social Security Institute, Pasaje San Lorenzo No. 205, Guayabamba, Iquitos, Perú | Division of Plant Sciences and Ecology, Regional Research Laboratory, Jorhat 785 006, Assam, India
Abstract: The leaf oils of Piper callosum from Peruvian Amazon and Michelia montana from Assam, India, were prepared by hydrodistillation and analyzed by a combination of GC and GC/MS. Twenty five and thirty components have been identified, representing 96.3 and 100.0% of the respective oils. The major constituents were found to be asaricin (syn. sarisan) (35.9 and 81.8%, respectively) and safrole (20.2 and 13.0%). The oil of P. callosum contained in addition eugenyl methyl ether (9.7%) and (E)‐asarone (7.8%), compounds not detected in the M. montana oil. The identity of the principal compound, an isomer of myristicin, was unequivocally established by ^{13}C‐NMR spectrometric techniques, especially long‐range ^{1}H–^{13}C correlation.
