Neurotrophic effect of submicrosomal fractions obtained from pre-degenerated peripheral nerves

Article type: Research Article

Authors: Lewin-Kowalik, Joanna | Barski, Jaroslaw-Jerzy | Krause, Mieczyslaw | Górka, Dariusz | Golka, Beata | Larysz-Brysz, Magdalena

Affiliations: Department of Physiology, Silesian Medical School, ul. Medyków 18, 40-762 Katowice, Poland

Note: [] Corresponding author.

Abstract: Submicrosomal fractions obtained from pre-degenerated distal stumps of sciatic nerves were implanted by means of connective tissue chambers into the injured hippocampus for 8 and 18 weeks. The nerve stumps were allowed to pre-degenerate for 7, 28 and 35 days. The neuronal outgrowth was examined by means of FITC-HRP injected into the chamber. Eight weeks postoperatively the greatest number of traced cells was present in brains treated with the fraction obtained from nerves pre-degenerated for 7 days. Eighteen weeks following implantation the greatest number of FITC-HRP positive cells was found in brains grafted with the fraction from nerves pre-degenerated for 35 days.

Keywords: Peripheral nerve pre-degeneration, Submicrosomal fraction, Neurite outgrowth, Hippocampus, Autologous connective tissue chambers

DOI: 10.3233/RNN-1994-7202

Journal: Restorative Neurology and Neuroscience, vol. 7, no. 2, pp. 71-78, 1994