Affiliations: Division of Neuropathology, Department of Pathology, The Ohio State University, Columbus, OH (USA)
Note: [] Correspondence: A.J. Yates, The Ohio State University, Department of Pathology, Division of Neuropathology, Room 111 E. Upham Hall, 473 W. 12th Avenue Columbus, OH 43210. Fax: (1) (614) 293-8223.
Abstract: PC12 cells grow in serum-free medium (SFM) if high doses of insulin (5–50 μg/ml), or combined insulin (5 μg/ml) and insulin-like growth factor-I (3 ng/ml) are added daily. Ganglioside GMl counteracts these effects in a dose-responsive fashion between 10 nM and 50 μM. This provides a serum-free system to study the interactive effects of gangliosides on growth and neuritogenesis in PC12 cells.
