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Article type: Research Article
Authors: Vigna, L.a | Novembrino, C.b | De Giuseppe, R.c | de Liso, F.c | Sommaruga, D.d | Agnelli, G.a | Belluigi, V.e | Riboldi, L.a | Bamonti, F.c
Affiliations: [a] Dipartimento Medicina Preventiva, del Lavoro e dell' Ambiente-Clinica del Lavoro L. Devoto, U.O. Medicina del Lavoro I, Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Via S. Barnaba 8, 20122 Milan, Italy. e-mail: luisella.vigna@policlinico.mi.it | [b] Dipartimento di Scienze Neurologiche, Università degli Studi di Milano, Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milan, Italy | [c] Dipartimento di Scienze Mediche, Università degli Studi di Milano, Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milan, Italy | [d] Servizio Dietetico Direzione Sanitaria di Presidio, Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milan, Italy | [e] Novelis SPA, Bresso, Italy
Abstract: Nutritional status, referred to meat and vegetable food consumption, is related to folate and B12 vitamin levels; hyperhomocysteinemia (HHcy) is due to folate and B12 vitamin–methionine metabolism imbalance, which can lead to oxidative stress, OS (imbalance between reactive oxygen species, ROS, and total antioxidant capacity, TAC). Possible early vitamin B12 (B12) deficiency can be highlighted by holotranscobalamin (HoloTC, bioactive cobalamin fraction) assay. Erythrocyte folate is a biomarker of 2- to 3-month folate storage. To evaluate nutritional status and OS we conducted an observational study on 118 occupational obese subjects (34M/84F, aged 16–69, median 46.5 years; BMI 26.5–54.1, median 33.3 kg/m2) without previous cardiovascular disease. Serum TAC and ROS (spectrophotometry, Diacron International, Italy), serum B12 and HoloTC, serum and erythrocyte folate, plasma Hcy (immunoenzymatic, AxSYM, Abbott Diagnostics, USA), lipid panel and inflammatory parameters by routine methods. All subjects showed adequate serum and erythrocyte folate levels, but HoloTC values revealed cobalamin deficiency in 30% of cases not congruent with B12 concentrations, low only in 10%. 43% of cases showed mild HHcy (>10.5 μmol/L; median 12.44 μmol/L, IQR 11.2–16.2). OS was found by normal mean TAC values (370.5 micromolHClO/mL, 340–405; n.v. > 350) but increased mean ROS concentrations (386 CarrU, 337–434; n.v. 250–300) in 93% of subjects. Normal folate and B12 vitamin levels revealed a good nutritional status in our occupational obese subjects. The presence of Oxidative Stress, due to imbalance between ROS and TAC and mild hyperhomocysteinemia may heighten the obesity-related cardiovascular risk.
Keywords: Homocysteine, Folate, Cobalamin, Nutrition, Lipid panel, Cardiovascular risk factors
DOI: 10.3233/s12349-010-0003-1
Journal: Mediterranean Journal of Nutrition and Metabolism, vol. 4, no. 1, pp. 69-74, 2011
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