Affiliations: [a] Master in Biomedical Sciences, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, North Sekip, Yogyakarta, Indonesia
| [b] Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, North Sekip, Yogyakarta, Indonesia
| [c] Department of Anatomical Pathology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, North Sekip, Yogyakarta, Indonesia
| [d] Department of Anatomical Pathology, Dr.Sardjito Hospital, Sekip, Yogyakarta, Indonesia
Correspondence:
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Corresponding author: Widya Wasityastuti; Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Farmako Street, North Sekip, Yogyakarta 55281, Indonesia. Tel.: +62 274 649 2492; Fax:+ 62 274 631185; E-mail: wasityastuti@ugm.ac.id.
Abstract: BACKGROUND:Inflammatory Bowel Disease (IBD) causes gastrointestinal tract dysregulation and triggers inflammation. Severe inflammatory events often lead to leukocyte infiltration. Yacon is known to have anti-inflammatory properties, making it a potential alternative treatment for colitis. AIMS:To investigate colonic Interleukin-23 (IL-23) level and leukocyte infiltration profile in 2,4,6-Trinitrobenzene Sulfonic Acid (TNBS)-induced colitis mouse model treated with yacon syrup powder. METHODS:Thirty male BALB/c mice (5 weeks old, body weight: 30.02±1.28 g) were randomly divided into 6 groups (K1-K6). Intra-rectal administration of TNBS was performed to K2-K6 to make the colitis model. K4-K6 were given yacon syrup powder daily for 14 days with the dose of 0.165, 0.331, and 0.662 g/30 g body weight (BW) respectively. IL-23 levels of colonic tissue were measured using ELISA. Leukocyte infiltration profile was calculated through microscopic observation of histology slides and presented in percentage and degree. RESULTS:Significantly different IL-23 levels were observed among the groups (P < 0.001). The percentage and degree of leukocyte infiltration in yacon-treated groups showed a significant decrease compared to colitis groups (P < 0.001). CONCLUSION:Yacon syrup powder provides beneficial effects on colitis mice by lowering the IL-23 level and number of leukocytes at the inflammation site.
