Customized bioresin formulation for stereolithography in tissue engineering

Article type: Research Article

Authors: Sakarya, Deniz^{a; b; 1} | Barlas, Firat Baris^{a; 2} | Sahin, Yesim Muge^{c; 3} | Yucel, Sevil^{b; 4; *}

Affiliations: [a] Istanbul University-Cerrahpaşa, Institute of Nanotechnology and Biotechnology, Istanbul, Turkey | [b] Department of Bioengineering, Yildiz Technical University, Faculty of Chemistry-Metallurgy, Istanbul, Turkey | [c] Istanbul Arel University, Polymer Technologies and Composite Application and Research Center (ArelPOTKAM), Buyukcekmece, Istanbul, Turkey

Correspondence: [*] Corresponding author: Sevil Yucel, Department of Bioengineering, Yildiz Technical University, Faculty of Chemistry-Metallurgy, Istanbul, Turkey. E-mail: yuce.sevil@gmail.com.

Note: [1] ORCID: https://orcid.org/0000-0003-0717-7637

Note: [2] ORCID: https://orcid.org/0000-0001-6401-686X

Note: [3] ORCID: https://orcid.org/0000-0003-2119-1216

Note: [4] ORCID: https://orcid.org/0000-0002-9495-9321

Abstract: Recently, advancements in fabrication technology have brought a new aspect to the field of tissue engineering. By utilizing advanced techniques in 3D manufacturing and biomaterials, scientists have successfully created tissue engineering scaffolds with complex three-dimensional structures and customized chemical compositions that closely mimic the natural environment of living tissues. These methodologies show potential not only for developing therapies that restore lost tissue function but also for creating in vitro models that replicate living tissue. The current investigation involved the synthesis of methacrylated polycaprolactone (PCLMA) by incorporating methacryloyl chloride (Meth-Cl) into polycaprolactone (PCL) with a molecular weight of 80,000 Da. Afterwards, PCLMA was subjected to crosslinking with glycerol acrylate (GA) and, by utilizing Diphenyl(2,4,6-trimethylbenzoyl) phosphine oxide (TPO) as a photoinitiator, achieved the three-dimensional (3D) printing of tissue materials using Stereolithography (SLA). Analytical techniques included nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Cell viability was investigated using Human Osteoblast (HOB) cells. The biocompatibility of glycerol acrylate (GA) crosslinked polymethacrylated polycaprolactone (PCLMA) was confirmed using cell viability experiments. Overall, the GA-crosslinked PCLMA bioresin, particularly PCLMA-8, shows promise for further use in tissue engineering applications.

Keywords: 3-D printing, Methacrylated Poly Caprolactone (PCLMA), tissue engineering, photopolymerization, stereolithograpy (SLA)

DOI: 10.3233/MGC-240002

Journal: Main Group Chemistry, vol. 23, no. 3, pp. 271-282, 2024