Effect of organic additives on storage stability of camel liver catalase against environmental conditions

Article type: Research Article

Authors: Zeyadi, Mustafa^{; *}

Affiliations: Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

Correspondence: [*] Corresponding author: Mustafa Zeyadi, Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia. E-mail: mzyadi@kau.edu.sa.

Abstract: The storage stability of catalase is very low under practical reaction conditions. Therefore, this study is aimed to evaluate the storage stability of catalase from camel liver by treated it with polyethylene glycol (PEG), glycerol, bovine serum albumin (BSA) and glucose against different storage conditions. The effect of some additives as stabilizers on the storage stability of camel liver catalase at 4°C and 30°C after 30 and 90 days was studied. The enzyme with additives at 5%concentration was more stable at 4 and 30°C for 90 days of incubation than native enzyme. The activity of catalase was more thermal stable in presence of 5%additive. The activity of enzyme with 5%BSA and glucose was retained 60%of its activity at 60°C. At pH 11 the catalase with glucose retained 30%of its activity. The native enzyme lost 80%of its activity in presence of 5 mM β-mercaptoethanol, where the enzyme with additive retained 30–46%of its activity. At 8 M urea, the native enzyme and enzyme with 5%of all additives retained 28%and 61–77%of its activity, respectively. The catalase with additives retained 50–90%of its activity in presence of different dyes. The results appeared that the catalase with additives may be used for elimination of excess of hydrogen peroxide after bleaching of textile.

Keywords: Camel, liver, catalase, additives, stability

DOI: 10.3233/MGC-210121

Journal: Main Group Chemistry, vol. 21, no. 1, pp. 225-231, 2022