Affiliations: Istituto di Matematica e Fisica, Università degli
Studi di Sassari, Sassari, Italy | Department of Bio-System Engineering, Faculty of
Engineering, Yamagata University, Yonezawa, Japan | Institute of Clinical Medicine, University of Tsukuba,
Tsukuba, Japan | Dipartimento di Fisica, Universita di Roma, Roma,
Italy
Note: [] Corresponding author: Donepudi V. Rao, Istituto di Matematica e
Fisica, Università degli Studi di Sassari, Via Vienna 2, Sassari 07100,
Italy. Tel.: +39 079 229 480; Fax: +39 79 229 482; E-mail: dvrao@uniss.it
Abstract: Synchrotron-based micro-CT was utilized to image the embedded
biological soft-tissue of a small animal shell. Micro-CT images of the
biological soft-tissue were acquired using 20, 25, and 27 keV synchrotron
X-rays with contrast agents, such as water, physiological saline and iodine.
Visualized the complex features of the animal at the above energies with water,
physiological saline and iodine. The choice of the optimum energy was chosen
based on the contrast mechanisms to know more about soft-matter and the
associated internal complex biological features of the small animal shell. This
way, the images at 27 keV (optimum energy) was reasonably acceptable for better
visualization of the interior micro-architecture, such as soft-anatomy,
physiology and internal organs of the animal with better visibility. The
introduction of water, physiological, or iodine as contrast agents is shown to
enhance minute image features in synchrotron-based tomographic imaging.
