Affiliations: Division of Neonatal Perinatal Medicine, University of
Texas Southwestern Medical Center, Dallas, TX 75390-9063, USA | Department of Pediatrics, Women & Infants
Hospital of Rhode Island/Brown Medical School, Providence, RI 02905, USA | Department of Pediatrics, Weil Medical College at
Cornell University, New York, NY 10021, USA | Departments of Pediatrics, The University of Texas
Southwestern Medical Center, Dallas, TX 75390-9063, USA
Note: [] Corresponding author: Lina F. Chalak, MD, Division of Neonatal
Perinatal Medicine, University of Texas Southwestern Medical Center, 5323 Harry
Hines Blvd, Dallas, TX 75390-9063, USA. E-mail: chalaklinaf@uams.edu
Abstract: A neonatal piglet model was used to determine IL6, IL8 and CRP
responses to asphyxia and subsequent volume infusion. Mechanically ventilated
swine (n=37, age: 8 ± 2 days, weight:
2.2 ± 0.7 kg) were progressively asphyxiated by changing
the ventilator gases until heart rate < 100 bpm and mean
arterial blood pressure < 20 mmHg. After 5 minutes of
ventilatory resuscitation, piglets were randomized to receive volume infusion
with either 5% Albumin (ALB), Normal Saline (NS), or no volume (SHAM). IL6,
IL8 and CRP were measured at baseline, at the end of asphyxia (ASPYHXIA), and 2
hrs after resuscitation (RESUSCITATION). IL6 (median and [25–75 percentiles]
pg/ml) increased from 163 [55,193] at baseline, to 263 [196,393] at ASPHYXIA,
and 441 [309,875] at RESUSCITATION (p<0.001). Both IL8 and
CRP were similar to baseline at all time intervals. Animals randomized to
volume (ALB and NS) vs. SHAM had higher IL6 levels following RESUSCITATION (749
[383,1163] vs. 303 [204,465], p<0.02). In this model of
severe neonatal asphyxia, serum IL-6 was elevated following volume infusion.
The lack of change in IL8 and CRP suggests selectivity in the immediate
inflammatory responses following asphyxia and volume resuscitation.
